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Error! Quality scores outside of set range #23

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Balakulandai opened this issue Dec 13, 2021 · 1 comment
Closed

Error! Quality scores outside of set range #23

Balakulandai opened this issue Dec 13, 2021 · 1 comment

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@Balakulandai
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Hi everyone
I run the command
./NGmerge -1 /home/planktonecology/Manuscript_Thatha/Metagenome_Thatha/CG_DN_935/AST5_R1.fastq.gz -2 /home/planktonecology/Manuscript_Thatha/Metagenome_Thatha/CG_DN_935/AST5_R2.fastq.gz -o AST5_merged.fastq.gz

I got error as follows
Error! Quality scores outside of set range

@KuechlerO
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KuechlerO commented Dec 14, 2021

Take a look here:
[(https://github.com//issues/4)]

More specifically:

I have the same issue, and it is not solved by setting '-u 41' by itself: The -w parameter must also be supplied with a modified qual_profile.txt. Modifying the matrixes with an extra row and column worked (I simply copied the outermost values).

@jsh58 jsh58 closed this as completed Feb 17, 2024
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