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Assertion error when trying to run with --resistance #69

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lknegendorf opened this issue May 4, 2023 · 0 comments
Open

Assertion error when trying to run with --resistance #69

lknegendorf opened this issue May 4, 2023 · 0 comments

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@lknegendorf
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I tried running Kleborate v2.3.2 on a LinusMint 21.1 system using the --resistance option and got an Assertion Error:

Traceback (most recent call last):
File "/home/nanopore/Kleborate/./kleborate-runner.py", line 22, in
main()
File "/home/nanopore/Kleborate/kleborate/main.py", line 64, in main
results.update(get_resistance_results(data_folder, contigs, args, res_headers,
File "/home/nanopore/Kleborate/kleborate/main.py", line 570, in get_resistance_results
res_hits = resblast_one_assembly(contigs, gene_info, qrdr, trunc, omp, seqs,
File "/home/nanopore/Kleborate/kleborate/resBLAST.py", line 32, in resblast_one_assembly
hits_dict = blast_against_all(seqs, min_cov, min_ident, contigs, gene_info,
File "/home/nanopore/Kleborate/kleborate/resBLAST.py", line 115, in blast_against_all
aa_result = check_for_exact_aa_match(seqs, hit, contigs)
File "/home/nanopore/Kleborate/kleborate/resBLAST.py", line 189, in check_for_exact_aa_match
assert hit_seq == gene_nucl_seq
AssertionError

I would be really happy about any suggestions how to fix this.

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