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Chaining script improvements: 5 merge, output fasta/fastq #2

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Magdoll opened this issue Mar 14, 2017 · 1 comment
Closed
3 tasks done

Chaining script improvements: 5 merge, output fasta/fastq #2

Magdoll opened this issue Mar 14, 2017 · 1 comment

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@Magdoll
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Magdoll commented Mar 14, 2017

Improvements to chain_samples.py

Top priority:

  • allow 5' merge, that is, if one isoform in sample A is a 5' truncted form of another in sample B, allow them to be chained as equivalent and select the longer form. Prerequisite: both sample A and B must have already gone through collapse using 5merge option, otherwise results can be incorrect.

  • output fasta/fastq files along with the current output of chained GFF, ids txt, and count.

Medium priority:

  • allow iterative chaining via pickled object
@Magdoll
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Magdoll commented Mar 14, 2017

5merge is now allowed via chain_samples.py --allow_5merge
fastq output is now supported via sample.config having an optional FASTQ_FILENAME=xxxx.

code updated to version 2.4.

tutorial available here

@Magdoll Magdoll closed this as completed Sep 27, 2017
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