Replies: 1 comment
-
The issue was resolved via support@milaboratories.com (something went wrong in the wet lab causing missing nucleotides of UMI; workaround was to analyze without UMIs) |
Beta Was this translation helpful? Give feedback.
0 replies
Sign up for free
to join this conversation on GitHub.
Already have an account?
Sign in to comment
-
Hello,
Could you help me understand why there is so low reads used in clonotypes ?
Reads used in clonotypes, percent of total: 13696 (1.73%)
We're working on human PBMC cultured in vitro. The sample isn't stimulated by Ag (Day 0).
For sequencing, the Takara SMART-Seq Human BCR IgG IgM IgA IgD IgE H/K/L Profiling Kit was used. We only used the primer for IgH
The alignment looks good and the overlapping too
Successfully aligned reads: 702545 (88.53%)
Overlapped: 661538 (83.36%)
Code used :
I attach the report and fastQC files via filesender:
123_J0_unstim.report.txt
https://filesender.renater.fr/?s=download&token=60478198-bf78-47cd-83db-caf1f722b35a
Thank you for your help
Beta Was this translation helpful? Give feedback.
All reactions