No such file or directory: 'Porter5/example/2FLGA.fasta.flatpsi.an

[katrinr]

Hi,
I would love to use Porter5, but I have some problems with the initial steps, which seems to be addressed in one or the other previous issues, however testing the path to the databases did not help, so I guess my paths are somehow not fully correct. Here is my config.ini

[DEFAULT]                                                                                                                               
psiblast = <path_to_packages>/packages/databases/psi-blast/ncbi-blast-2.8.1+                                                                
uniref90 = <path_to_packages>/packages/databases/uniref90                                                                                   
hhblits = <path_to_packages>/packages/hh-suite                                                                                              
uniprot20 = <path_to_packages>/packages/databases/uniprot20_2016_02

How should it look like?
Thanks in advance

[katrinr]

Figured it out, maybe useful for others, so I post it here

[DEFAULT]                                                                                                                               
psiblast = <path_to_packages>/packages/databases/psi-blast/ncbi-blast-2.8.1+                                                                
uniref90 = <path_to_packages>/packages/databases/uniref90/uniref90.fasta                                                                    
hhblits = <path_to_packages>/packages/hh-suite/build/bin/hhblits                                                                            
uniprot20 = <path_to_packages>/packages/databases/uniprot20_2016_02/uniprot20_2016_02 

Btw thanks for the tool!

[mircare]

HHblits is a bit confusing in this respect so I've just improved the description of uniprot20.

Thank you for using Porter5!

[DRL36]

I encountered the same issue here and I've tried changing the default, but it still went wrong. For me, 2FLGA.fasta.flatpsi is created, but no 2FLGA.flatpsi.ann file. Any ideas on how to fix this?
btw, I commented out psiblast and uniref90 since I'm running the fast prediction and those 2 are not needed.

[mircare]

@DRL36 Please try the below command and open another issue with more details if the problem persists.

python3 Porter5.py -i example/2FLGA.fasta --cpu 4 --setup