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`
STATUS: Running VEP and writing to: /home/gabriel.gama/Mutect2_usingPoN/sample11_withPoN.vep.vcf
Can't locate Bio/EnsEMBL/VEP/Runner.pm in @inc (you may need to install the Bio::EnsEMBL::VEP::Runner module) (@inc contains: /home/gabriel.gama/miniconda3/bin/modules /home/gabriel.gama/miniconda3/bin /usr/lib/perl5/5.34/site_perl /usr/share/perl5/site_perl /usr/lib/perl5/5.34/vendor_perl /usr/share/perl5/vendor_perl /usr/lib/perl5/5.34/core_perl /usr/share/perl5/core_perl) at /home/gabriel.gama/miniconda3/bin/vep line 20.
BEGIN failed--compilation aborted at /home/gabriel.gama/miniconda3/bin/vep line 20.
Hello everyone! I'm trying to run VCF after sucessfully using VEP.
When I use:
`
vcf2maf --input-vcf /home/gabriel.gama/Mutect2_usingPoN/sample11_withPoN.vcf --output-maf /home/gabriel.gama/Mutect2_usingPoN/sample11_vcf2maf.vep.maf --ref-fasta /home/gabriel.gama/references/ENSEMBL/star/Homo_sapiens.GRCh38.dna.primary_assembly.fa
`
I'm getting:
`
STATUS: Running VEP and writing to: /home/gabriel.gama/Mutect2_usingPoN/sample11_withPoN.vep.vcf
Can't locate Bio/EnsEMBL/VEP/Runner.pm in @inc (you may need to install the Bio::EnsEMBL::VEP::Runner module) (@inc contains: /home/gabriel.gama/miniconda3/bin/modules /home/gabriel.gama/miniconda3/bin /usr/lib/perl5/5.34/site_perl /usr/share/perl5/site_perl /usr/lib/perl5/5.34/vendor_perl /usr/share/perl5/vendor_perl /usr/lib/perl5/5.34/core_perl /usr/share/perl5/core_perl) at /home/gabriel.gama/miniconda3/bin/vep line 20.
BEGIN failed--compilation aborted at /home/gabriel.gama/miniconda3/bin/vep line 20.
ERROR: Failed to run the VEP annotator! Command: /usr/bin/perl /home/gabriel.gama/miniconda3/bin/vep --species homo_sapiens --assembly GRCh37 --no_progress --no_stats --buffer_size 5000 --sift b --ccds --uniprot --hgvs --symbol --numbers --domains --gene_phenotype --canonical --protein --biotype --uniprot --tsl --variant_class --shift_hgvs 1 --check_existing --total_length --allele_number --no_escape --xref_refseq --failed 1 --vcf --flag_pick_allele --pick_order canonical,tsl,biotype,rank,ccds,length --dir /home/gabriel.gama/.vep --fasta /home/gabriel.gama/references/ENSEMBL/star/Homo_sapiens.GRCh38.dna.primary_assembly.fa --format vcf --input_file /home/gabriel.gama/Mutect2_usingPoN/sample11_withPoN.vcf --output_file /home/gabriel.gama/Mutect2_usingPoN/sample11_withPoN.vep.vcf --offline --pubmed --fork 4 --polyphen b --af --af_1kg --af_esp --af_gnomad --regulatory
`
Any ideas?
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