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How does this match barcodes #4

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dlzurich opened this issue May 15, 2014 · 0 comments
Open

How does this match barcodes #4

dlzurich opened this issue May 15, 2014 · 0 comments

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@dlzurich
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Hi, I am wondering what method this software uses to match the read barcodes to that provided? I am running it with different levels of mismatching (0,1, 2), but when I go from 1 to 2 the allocation of reads to certain barcodes is decreasing. My barcodes have been designed to be identifiable with up to 3 mismatches, so I was curious what could cause this and was wondering how the software selects the "correct" barcode if you allow mismatches?

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