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Hi, I am wondering what method this software uses to match the read barcodes to that provided? I am running it with different levels of mismatching (0,1, 2), but when I go from 1 to 2 the allocation of reads to certain barcodes is decreasing. My barcodes have been designed to be identifiable with up to 3 mismatches, so I was curious what could cause this and was wondering how the software selects the "correct" barcode if you allow mismatches?
The text was updated successfully, but these errors were encountered:
Hi, I am wondering what method this software uses to match the read barcodes to that provided? I am running it with different levels of mismatching (0,1, 2), but when I go from 1 to 2 the allocation of reads to certain barcodes is decreasing. My barcodes have been designed to be identifiable with up to 3 mismatches, so I was curious what could cause this and was wondering how the software selects the "correct" barcode if you allow mismatches?
The text was updated successfully, but these errors were encountered: