01. Downloading SRA Toolkit

NCBI SRA Toolkit

Below are the latest releases of various tools and release checksum file.

SRA Toolkit

Compiled binaries/install scripts of August 29, 2023, version 3.0.7:

• CentOS Linux 64 bit architecture - non-sudo tar archive
• Ubuntu Linux 64 bit architecture - non-sudo tar archive
• Cloud - apt-get install script - for Debian and Ubuntu - requires sudo permissions
• Cloud - yum install script - for CentOS - requires sudo permissions
• MacOS 64 bit architecture
• MS Windows 64 bit architecture
• Docker image repository
• md5 checksums

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Magic-BLAST

Magic-BLAST is a tool for mapping large next-generation RNA or DNA sequencing runs against a whole genome or transcriptome

• Magic-BLAST executables for LINUX, MacOSX, and Windows as well as the source files are available on the FTP site
• Read more about Magic BLAST on the FTP site

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Third Party Software

Builds of Third Party Software Tools with SRA support:

• HISAT2 version 2.2.1-ngs.3.0.7 - graph-based alignment of next generation sequencing reads to a population of genomes with direct support of SRA, built for:
  • Linux 64 bit architecture

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Latest Source Code

• NCBI VDB Software Development Kit – August 29, 2023, version 3.0.7 release
• NCBI SRA Toolkit – August 29, 2023, version 3.0.7 release
• NCBI NGS Toolkit – August 29, 2023, version 3.0.7 release

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File checksums

You may validate downloaded files with md5 checksums computed using md5sum -b

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Note

The NGS SDK releases are in (https://github.com/ncbi/sra-tools/wiki/09.-Downloading-NGS-SDK)