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zipped fasta file doesn't work #277

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rodriguesk opened this issue May 19, 2023 · 3 comments
Closed

zipped fasta file doesn't work #277

rodriguesk opened this issue May 19, 2023 · 3 comments
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@rodriguesk
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Description of the bug

I’m getting this error and I’m wondering how to solve it. I’m using the genome mm39 (which is new and not in igenome) so I’m trying to provide custom genome to the pipeline, I’m getting the following error and I noticed that it is fixed when I unzip the fasta file.

Command used and terminal output

Error executing process > 'NFCORE_ATACSEQ:ATACSEQ:PREPARE_GENOME:CUSTOM_GETCHROMSIZES (null)'

Caused by:
  Not a valid path value type: groovyx.gpars.dataflow.DataflowVariable (DataflowVariable(value=null))

Relevant files

In the config file I’m using these in the params section:
fasta = '/oak/stanford/groups/smontgom/kameronr/methylseq/JG97/genomes/GRCm39_fasta/GRCm39.genome.fa.gz'
bwa_index = '/oak/stanford/groups/smontgom/kameronr/methylseq/JG97/genomes/GRCm39_fasta/'
gtf = '/oak/stanford/groups/smontgom/kameronr/methylseq/JG97/genomes/GRCm39_gtf/Mus_musculus.GRCm39.106.gtf'
macs_gsize = 2728222451

System information

No response

@rodriguesk rodriguesk added the bug Something isn't working label May 19, 2023
@sergpolly
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exact same here.
crashes with the error message above when fasta is compressed, proceeds beautifully when fasta is a text file ...
Have to admit it wasn't easy to debug - as all of the work folders are empty and .nextflow.log isn't very informative in this case

@JoseEspinosa JoseEspinosa added this to the 2.1 milestone May 29, 2023
bjlang pushed a commit to bjlang/nf-core-atacseq that referenced this issue Jun 2, 2023
@JoseEspinosa
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Should be fixed now in dev in case you want to give it a try.

@JoseEspinosa
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Will close this issue now, feel free to reopen it if you find any further issue.

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