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BarryDigby committed Feb 9, 2023
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Expand Up @@ -169,7 +169,7 @@ The pipeline is built using [Nextflow](https://www.nextflow.io/) and processes d

The workflow is designed to output three files _per sample_, _per quantification tool_ in the `circrna_discovery` directory. Using the test dataset as an example, the directory structure for the `fust_1` sample is:

```console
````console
|-- results/
|-- circrna_discovery/
|-- circexplorer2/
Expand All @@ -194,7 +194,7 @@ Sample outputs for the corresponding `.log`, `.bed` and `.fasta` entry are given
[nf-core/circrna]: Attempting to fit circRNA to gene exon boundaries
[nf-core/circrna]: chrI:1140805-1147588:- fits gene exons, is a circRNA
[nf-core/circrna]: cleaning up intermediate files
```
````

```console
chrI 1140805 1147588 chrI:1140805-1147588:- 2 - 1140805 1147588 0 5 229,214,191,141,499 0,1282,2546,4912,6284 circRNA Y48G8AL.10 NM_001306296,NM_001306297,NM_001306298,NM_001306299 1274
Expand All @@ -215,6 +215,7 @@ Intermediate files generated by each quantification tool are described in depth
<summary>Output files</summary>

- `circrna_discovery/circexplorer2/intermediates/${sample_id}/`

- `*.bed`: Intermediate file generated by `CIRCexplorer2 parse` module, identifying STAR fusion junctions for downstream annotation.
- `*_circexplorer2_circs.bed`: Filtered BED6 file containing circRNA counts used for count matrix generation.
- `*.txt`: Output files generated by `CIRCexplorer2 annotate` module, based on BED 12 format containing circRNA genomic location information, exon cassette composition and an additional 6 columns specifying circRNA annotations. Full descriptions of the 18 columns can be found in the `CIRCexplorer2` [documentation](https://circexplorer2.readthedocs.io/en/latest/modules/annotate/#output).
Expand Down Expand Up @@ -247,6 +248,7 @@ Intermediate files generated by each quantification tool are described in depth
<summary>Output files</summary>

- `circrna_discovery/circrna_finder/intermediates/${sample_id}/`

- `*.filteredJunctions.bed`: A bed file with **all** circular junctions found by the pipeline. The score column indicates the number reads spanning each junction.
- `*.s_filteredJunctions.bed`: A bed file with those junctions in `*.filteredJunctions.bed` that are flanked by GT-AG splice sites. The score column indicates the number reads spanning each junction.
- `*.s_filteredJunctions_fw.bed`: A bed file with the same circular junctions as in file (b), but here the score column gives the average number of forward spliced reads at both splice sites around each circular junction.
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