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--mirtrace_protocol arguments redundant, --protocol only should be sufficient #168

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gpalidwor opened this issue Jun 29, 2022 · 3 comments
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enhancement New feature or request

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@gpalidwor
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Description of feature

Currently smrna requires both --protocol and --mirtrace_protocol to be set. Both default to illumina. If --protocol is set to a non illumina value (e.g. nextflex) --mirtrace_protocol must be set to the same. As such the --mirtrace_protocol argument is redundant and can lead to confusing results if not set to match --protocol. I can think of no cases where --protocol and --mirtrace_protocol should be different

@gpalidwor gpalidwor added the enhancement New feature or request label Jun 29, 2022
@apeltzer apeltzer added this to the Release 2.1.0 milestone Jun 29, 2022
@apeltzer
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So when I have a look at the code, it has a flag that sets mirtrace_protocol to params.protocol whenever mirtrace_protocol has not been set.

@gpalidwor
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Thanks for looking at it; My experience was the mirTrace output was not meaningful (didn't map to anything) for --protocol nextflex until I also set --mirtrace_protocol nextflex so perhaps it's protocol specific..?

@apeltzer
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Fixed in dev

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