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[Feat] Add UMI Handling to the pipeline #164

Merged
merged 30 commits into from
Jan 11, 2024

Commits on May 10, 2022

  1. REVERT CHANGES

    Reverting changes to a non-linted version and added the umitools modules.
    Christian Kubica authored and Christian Kubica committed May 10, 2022
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  2. INCLUDE UMITOOLS WORKFLOW

    Added the umitools workflow and integrated it into the smrnaseq workflow
    Christian Kubica authored and Christian Kubica committed May 10, 2022
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Commits on May 13, 2022

  1. ADD DOCUMENTATION

    Add additional documentation to use UMI tools as part of the pipeline.
    Most of the documentation has been copied from nf-core/rnaseq.
    Christian Kubica authored and Christian Kubica committed May 13, 2022
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Commits on Jun 13, 2022

  1. ADD SAMTOOLS BAM2FQ MODULE

    The bam2fq module is neccessary to convert the deduplicated bam
    files back into a fastq format to be fed into the existing
    pipeline.
    Christian Kubica authored and Christian Kubica committed Jun 13, 2022
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Commits on Jun 14, 2022

  1. ADD UMITOOLS EXTRACT ARGS

    Added the umitools extract modules.config lines from nf-core/rnaseq
    to this pipeline.
    Christian Kubica authored and Christian Kubica committed Jun 14, 2022
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Commits on Jun 15, 2022

  1. UPDATE MODULES.CONFIG

    Added configurations for umi deduplication.
    Christian Kubica authored and Christian Kubica committed Jun 15, 2022
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  2. INCLUDE UMITOOLS DEDUP WORKFLOW

    Initial comit of the umi dedup subworkflow. The workflow combines
    already existing modules of the pipeline and nf-core module to
    deduplicate the reads by mapping them to the species genome and
    re-converting them to fastq after deduplication.
    Christian Kubica authored and Christian Kubica committed Jun 15, 2022
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  3. INCLUDE UMITOOLS DEDUP

    includes the optional umitools deduplication step after the read
    QC.
    Christian Kubica authored and Christian Kubica committed Jun 15, 2022
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  4. ADD SAMTOOLS SORT CONFIG

    Added additional configuration to change the output file name of
    samtools sort.
    CKComputomics committed Jun 15, 2022
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  5. FIX TYPO

    CKComputomics committed Jun 15, 2022
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  6. ADD DEDUP DOCUMENTATION

    Added the documentation detailing the output files of the UMI-tools
    deduplication step.
    CKComputomics committed Jun 15, 2022
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  7. ADD DEDUP STEP

    CKComputomics committed Jun 15, 2022
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  8. ADD UMITOOLS VERSION

    CKComputomics committed Jun 15, 2022
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Commits on Jun 20, 2022

  1. MERGE DEDUPLICATED AND UNMAPPED READS AFTER DEDUPLICATION

    After deduplication the reads that remained unaligned to the
    provided reference genome are merged with the set of deduplicated
    reads to enable the use of the full spectrum of reads, independent
    of potential reference bias. This behaviour can be deactivated by
    setting --umi_merge_unmapped false
    CKComputomics committed Jun 20, 2022
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  2. ADD MISSING OPTION

    CKComputomics committed Jun 20, 2022
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  3. ADD NEWLINE

    CKComputomics committed Jun 20, 2022
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Commits on Jun 21, 2022

  1. CLEAN CODE

    CKComputomics committed Jun 21, 2022
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  2. ADD DOCUMENTATION

    CKComputomics committed Jun 21, 2022
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  3. Configuration menu
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  4. FINALIZE DOCUMENTATION

    Information on the new --umi_merge_unmapped command were added to
    both the CHANGELOG, as well as the output markdown script.
    CKComputomics committed Jun 21, 2022
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  5. UPDATE MAIL TEMPLATE

    CKComputomics committed Jun 21, 2022
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  6. Configuration menu
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  7. PLEASE PRETTIER

    CKComputomics committed Jun 21, 2022
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  8. Configuration menu
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  9. FIX MERGE ERROR

    CKComputomics committed Jun 21, 2022
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  10. MAKE PRETTIER HAPPY

    CKComputomics committed Jun 21, 2022
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Commits on Jun 22, 2022

  1. ADD NF-CORE CAT

    Includes the nf-core cat module to replace the custom concatenation
    module.
    CKComputomics committed Jun 22, 2022
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  2. REPLACE CUSTOM CAT WITH NF-CORE CAT

    Implements the use of the nf-core cat module.
    CKComputomics committed Jun 22, 2022
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  3. REMOVE UNUSED MODULE

    deletes the now unused conatenation module.
    CKComputomics committed Jun 22, 2022
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Commits on Jan 11, 2024

  1. Configuration menu
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