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[Feat] Add UMI Handling to the pipeline #164
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Commits on May 10, 2022
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Reverting changes to a non-linted version and added the umitools modules.
Christian Kubica authored and Christian Kubica committedMay 10, 2022 Configuration menu - View commit details
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Added the umitools workflow and integrated it into the smrnaseq workflow
Christian Kubica authored and Christian Kubica committedMay 10, 2022 Configuration menu - View commit details
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Commits on May 13, 2022
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Add additional documentation to use UMI tools as part of the pipeline. Most of the documentation has been copied from nf-core/rnaseq.
Christian Kubica authored and Christian Kubica committedMay 13, 2022 Configuration menu - View commit details
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Commits on Jun 13, 2022
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The bam2fq module is neccessary to convert the deduplicated bam files back into a fastq format to be fed into the existing pipeline.
Christian Kubica authored and Christian Kubica committedJun 13, 2022 Configuration menu - View commit details
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Commits on Jun 14, 2022
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Added the umitools extract modules.config lines from nf-core/rnaseq to this pipeline.
Christian Kubica authored and Christian Kubica committedJun 14, 2022 Configuration menu - View commit details
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Commits on Jun 15, 2022
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Added configurations for umi deduplication.
Christian Kubica authored and Christian Kubica committedJun 15, 2022 Configuration menu - View commit details
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INCLUDE UMITOOLS DEDUP WORKFLOW
Initial comit of the umi dedup subworkflow. The workflow combines already existing modules of the pipeline and nf-core module to deduplicate the reads by mapping them to the species genome and re-converting them to fastq after deduplication.
Christian Kubica authored and Christian Kubica committedJun 15, 2022 Configuration menu - View commit details
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includes the optional umitools deduplication step after the read QC.
Christian Kubica authored and Christian Kubica committedJun 15, 2022 Configuration menu - View commit details
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Added additional configuration to change the output file name of samtools sort.
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Added the documentation detailing the output files of the UMI-tools deduplication step.
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Commits on Jun 20, 2022
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MERGE DEDUPLICATED AND UNMAPPED READS AFTER DEDUPLICATION
After deduplication the reads that remained unaligned to the provided reference genome are merged with the set of deduplicated reads to enable the use of the full spectrum of reads, independent of potential reference bias. This behaviour can be deactivated by setting --umi_merge_unmapped false
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Commits on Jun 21, 2022
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Information on the new --umi_merge_unmapped command were added to both the CHANGELOG, as well as the output markdown script.
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Commits on Jun 22, 2022
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Includes the nf-core cat module to replace the custom concatenation module.
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REPLACE CUSTOM CAT WITH NF-CORE CAT
Implements the use of the nf-core cat module.
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deletes the now unused conatenation module.
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Commits on Jan 11, 2024
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