This release (v0.3) of the Normalizer contains the following updates:
- The 'Event_length' channel in CyTOF2 data is no longer being normalized. Previously the Normalizer was excluding the 'Cell_length' channel in CyTOF1 data but not the equivalent channel with the updated 'Event_length' label in the CyTOF2.
- The bead singlet events identified before normalization, and the events removed after normalization, are saved in separate folders.
- The bug that prevented users from being able to select previously created files of beads to use as a normalization baseline has been fixed.
- The source code now includes p-code for several helper functions that were previously omitted.
- Closing the figure window gives the user an option to quit mid-normalization.
- The FCS reader and writer needed to run the Normalizer using the source code within Matlab are now available in the MatlabCytofUtilities repository. Make sure to use the versions there since they have several updates and fixes as compared to other versions. (They are still automatically included in the standalone application so no need to download them if you are using the MCR.)
For instructions about installing and running the Normalizer, see the wiki.
To learn more about normalization of mass cytometry data or to cite the Normalizer, see the article in Cytometry.