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DRB1-3123 example not producing a nice graph anymore after biwflambda update. #232

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subwaystation opened this issue Sep 20, 2022 · 5 comments

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@subwaystation
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Using the Dockerfile from #231 I get quite an ugly DRB1-3123 graph:

DRB1-3123 fa gz b1e1a2d 417fcdf a8de103 smooth final og lay draw_multiqc

docker pull ghcr.io/pangenome/pggb:latest
docker run -it -v ${PWD}/data/:/data ghcr.io/pangenome/pggb:latest "pggb -i /data/HLA/DRB1-3123.fa.gz -p 70 -s 3000 -G 2000 -n 12 -t 16 -o /data/bi_wfmash_test -M -m"
@ekg
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ekg commented Sep 22, 2022

What happens with default parameters?

@subwaystation
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docker run -it -v ${PWD}/data/:/data ghcr.io/pangenome/pggb:latest "pggb -i /data/HLA/DRB1-3123.fa.gz -n 12 -t 16 -o /data/bi_wfmash_test_1 -M -m

Other fancy graphs.

@subwaystation
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Alright, I got a nice one with this:

docker run -it -v ${PWD}/data/:/data ghcr.io/pangenome/pggb:latest "pggb -i /data/HLA/DRB1-3123.fa.gz -p 70 -s 2000 -G 2000 -n 12 -t 16 -o /data/bi_wfmash_test_2 -M -m"

DRB1-3123 fa gz c666522 417fcdf a8de103 smooth final og lay draw_multiqc

A shorter segment length was required. I am happy now.

@ekg
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ekg commented Sep 22, 2022

I'd suggest removing -G... that part is very different now. We actually iterate with very short -G three times. So we're just doing very local smoorthing now.

@subwaystation
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Not sure this helped. The tip of the cavallo seems worse.

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