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The query sequence returned by '%s' is not the same of the original one. #12

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xjllh opened this issue Feb 22, 2024 · 1 comment
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@xjllh
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xjllh commented Feb 22, 2024

The sequence cannot be displayed in the SEQUENCE ID and SEQUENCES sections after submitting the selected sequence when running NCBI BLAST using pymod 3.0.2. Simultaneously, an error occurs in PyMol, and the traceback is as follows:
Traceback (most recent call last):
File "D:\pymol\pymol_edu\pymol_edu_install\lib\site-packages\pmg_tk\startup\pymod3\pymod_lib\pymod_protocols\similarity_searches_protocols_base_blast.py", line 879, in
self.main_button.clicked.connect(lambda a=None: self.protocol.blast_results_state())
File "D:\pymol\pymol_edu\pymol_edu_install\lib\site-packages\pmg_tk\startup\pymod3\pymod_lib\pymod_protocols\similarity_searches_protocols_base_blast.py", line 445, in blast_results_state
self.import_results_in_pymod()
File "D:\pymol\pymol_edu\pymol_edu_install\lib\site-packages\pmg_tk\startup\pymod3\pymod_lib\pymod_protocols\similarity_searches_protocols_base_blast.py", line 583, in import_results_in_pymod
raise Exception("The query sequence returned by '%s' is not the same of the original one." % self.blast_version)
Exception: The query sequence returned by 'blast' is not the same of the original one.
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@irisdisilverio
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Could you tell me which sequence did you use?
To solve the issue, try performing remote BLAST using SwissProt as database or try running the BLAST locally, since there might be an issue with the NCBI server.

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