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The metadata.yaml following the import of the raw sequences, and the metadata.yaml following demux, indicate the data are phred 33. However, that does not appear to be accurate as the character set used includes characters defined outside of phred 33 encoding (e.g., "["):
$ funzip sequences.fastq.gz | head
@HWI-EAS440_0386:1:23:17547:1423#0/1
TACGNAGGATCCGAGCGTTATCCGGATTTATTGGGTTTAAAGGGAGCGTAGATGGATGTTTAAGTCAGTTGTGAAAGTTTGCGGCTCAACCGTAAAATTGCAGTTGATACTGGATATCTTGAGTGCAGTTGAGGCAGGGGGGGATTGGTGTG
+
hhhdHddddddddfehhfhhhghggfhhhfhhgggfhhgfgdfcfhehfdgfhggfggfggffgddfgdffdgdaagaaddcbdccc]a^ad__a]_____ba_`a`__^__\]^OWZR\Z\\WYTZ_U^BBBBBBBBBBBBBBBBBBBBBB
I checked with @gregcaporaso and he indicated that it made sense given the age of the data.
At the time of creation of this issue, I do not believe there is a functional impact with the inaccuracy of metadata.yaml as quality scores are not interrogated explicitly by Q2 in this tutorial.
The text was updated successfully, but these errors were encountered:
The
metadata.yaml
following the import of the raw sequences, and themetadata.yaml
following demux, indicate the data are phred 33. However, that does not appear to be accurate as the character set used includes characters defined outside of phred 33 encoding (e.g., "["):I checked with @gregcaporaso and he indicated that it made sense given the age of the data.
At the time of creation of this issue, I do not believe there is a functional impact with the inaccuracy of
metadata.yaml
as quality scores are not interrogated explicitly by Q2 in this tutorial.The text was updated successfully, but these errors were encountered: