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Overlapper SE file has no IDs and quality strings appear to be wrong and/or made up of sequences #39

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samhunter opened this issue Dec 28, 2016 · 4 comments

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@samhunter
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@id
ATGCTCACTACCACTCTCTCCGTACTGGAATATTCATCTACTCCCATTTTGCTGACTCTTATACCTCTCAGCGACATTTCCGTGCTTGGGGTCATATCAGGCAATATTCCGATCATTCCCATTACACTGTCAATGGGTTCAATCCCCCAGTTCTGGAAAAGCACTTTTGCATCCTTTTGGAAATGCCTCAAGAGTTGGTG
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@id
TTTCCCCAACGATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAAGTTTCTAATCGATTAAAGATCACACTGAAGTTCGCTTTCAGTATAATGTTCTTTTCCATGATCGCCTGGTCCATTCGCACATAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGCTATGT
+
"IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@id
NCGCCAAATATGCCTCTAGTTTGTTTCTCTGGCACATTCCGCATTCCTGTTGCCAATTTCAGAGTGTTTTGCTTAACATATCTGGGACAGACCCCATATGTGATCCTGTTTACATTTTGAAAGGGTTTGTCATTGGGAATGCTTCCATTTGGAGTAATGCATTCAGATTTGCAATTGACAATGGGTGCATCTGATCTCATTATTGAGCTTTTCCCACTCTGTATTTTGAAGTAACCCCGAGGGGCAATTAGATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTATTGT
+
#<0<BHIHHHHEGHHEHHHI?GGHIIEEHIE1DHHIIFHHFCDHEHEH7III4IIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII=;GI<<IIGIIIIIIATTCCCTGTGCTGTTAATCAAAAGTATGTCTCCCGGTTTTACTATTGT
@id
GGGCACCCCAGCTCAATCCTATTGATGGACCACTACCTGAAGATAATGAACCAAGTGGGTATGCACAAACAGACTGTGTCCTGGAGGCCATGGCTTTCCTTGAAGAATCCCACCCAGGGATATTTGAGAATTCATGCCTTGAAACAATGGAAATTGTCCAACAAGCAAGGGTGGATAAACTAACTCAGGGTCGCCAGACTTATGATTGGACATTAAATAGAAATCAACCGGCAGCAACTGCATTGGCCAACACCATAGAAGTTTTTAGATCAAATGGTCTCACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAAGATGTAATGGAATCAATGGATAAAGAGGAAATAGAGATAACAACACACTTTCAAAGAAAAAGGAGAGTAAGGGATAACATGACCAAGAAGATGGTCACACAA
+
DCDDDIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIHHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIHIIIHIIIIIIIIIIIIIHIIIIIIIIIIIIIIIIIGIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIACCATAGAAGTTTTTAGATCAAATGGTCTCACAGCTAATGAGTCAGGAAGGCTAATAGATTTCTTAAAAGATGTAATGGAATCAATGGATAAAGAGGAAATAGAGATAACAACACACTTTCAAAGAAAAAGGAGAGTAAGGGATAACATGACCAAGAAGATGGTCACACAA
@id
AATGTACTCAAATGCAAATGTTGCACCTAATGTTGCCTTTTTGGCAGGCCCACATAATGAACCCCAGCAGAACAACACAAAGCAAAAAGCATGACATGGCAAAGGAAATCCATAGGATCCAATCTTTGTATCCTGACTTCAGCTGAACACCTTTGATCTGGAACCGATTGCTTAATGCCTCGTCTCTGTATAC
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@id
CCTCTGCTGCTTGTTCACTTGACCCAGCCATTTGTTCCATGGCCTTAGCTGTTGTGCTGGCTATTACCATTCTGTTCTCGTGCCTGATTAGTGGATTGGTTGTTGTCACCATTTGTCTATGAGATCGATGCTGGGAATCAGCAATCTGTTCACAGGTTGCGCATACCAGACCAAATGCCACCTCAGTGGCGACAGTCCCCATTCTGTTGTATAT
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@id
NCAGAATGCCCTCAATGGGAATGGTGACCCAAACAACATGGACAAGGCAGTCAAACTGTATAGGAAACTAAAACGAGAAATAACATTCCATGGGGCCAAAGAAGTAGCACTCAGTTATTCTGCCGGTGCACTTGCCAGTTGCATGGGCCTCATATACAACAGAATGGGGACTGTCGCCACTGAGGTGGCATTTGGTCTGGTATGCGCAACCTGTGAACAGATTGCTGATTCCCAGCATCGATCTCATAGACAAATGGTGACAACAACCAATCCACTAATCAGGCACGAGAACAGAATGGTAATAGCCAGCACAACAG
+
#<DDDIGHHHHIIIIIHHIIHIHHIEHIHIHHIIIIHHHIHIIIIHIIIHCEHIHHHIHHIHIIHHIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII"AAATGGTGACAACAACCAATCCACTAATCAGGCACGAGAACAGAATGGTAATAGCCAGCACAACAG
@id

@samhunter
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It is difficult to see in the text I pasted above, but view it fixed-width in a txt editor. It seems like the last piece of the quality string is the read!

dstreett added a commit that referenced this issue Dec 28, 2016
@dstreett
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Copy paste error

finalQual += seq2.substr(maxLoop, r2.getLength() - maxLoop);

Should have been . . .

finalQual += qual2.substr(maxLoop, r2.getLength() - maxLoop);

@samhunter samhunter reopened this Dec 30, 2016
@samhunter
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ID text still blank:

@id
ATGCTCACTACCACTCTCTCCGTACTGGAATATTCATCTACTCCCATTTTGCTGACTCTTATACCTCTCAGCGACATTTCCGTGCTTGGGGTCATATCAGGCAATATTCCGATCATTCCCATTACACTGTCAATGGGTTCAATCCCCCAGTTCTGGAAAAGCACTTTTGCATCCTTTTGGAAATGCCTCAAGAGTTGGTG
+
IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII
@id
TTTCCCCAACGATTGCTCCCTCCTCAGTGAAAGCCCTTAGTAGTATCAAAGTTTCTAATCGATTAAAGATCACACTGAAGTTCGCTTTCAGTATAATGTTCTTTTCCATGATCGCCTGGTCCATTCGCACATAAAGAGGGCCTATTATCTTTTGCCTAGGCATGAGCATGAACCAGTCTCGTGACATTTCCTCGAGGGTCATGTCAGCTATGT
+
"IIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIIII

@dstreett
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dstreett commented Jan 2, 2017

It is a feature to reduce memory. ;) Just kidding, Fixed in N-Remover, will close once merged into master.

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