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sadaf #154
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Hi, On 22 June 2015 at 10:08, Safmughal notifications@github.com wrote:
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Hi, parent, Graph::Writer::Dot, Sub::Exporter, Eval::Closure, Devel::OverloadInfo, Package::DeprecationManager, B::Hooks::EndOfScope, Package::Stash, namespace::clean, Module::Implementation, Data::OptList, Class::Load, Moose, Moose::Role, Test::Files, Graph::Writer, File::Which, Log::Log4perl, Text::CSV, Graph, File::Slurp::Tiny, File::Grep, File::Find::Rule, Exception::Class, Array::Utils Some of the perl modules had to forcibly installed due to test errors. My final observation is that there doesn't appear to be a "BEDTOOLS_LIB_DIR", however I'm not sure why. I hope the above details help. |
Hi Sadaf, With regards to the perl modules, 'cpanm Bio::Roary' should install all the Thanks for the feedback. On 22 June 2015 at 11:36, Safmughal notifications@github.com wrote:
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Hi Sadaf, brew tap homebrew/science brew install bedtools cd-hit blast mcl prank parallel mafft exonerate cpanm -f Bio::Roary Regards, Andrew On 22 June 2015 at 13:13, Andrew Page andrewjpage@gmail.com wrote:
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Thanks Andrew for your efforts. I appreciate that. I will update you once I will go through it. |
Hi Andrew, Kind Regards |
Thanks for letting me know. On 23 June 2015 at 09:36, Safmughal notifications@github.com wrote:
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Hi. I downloaded Roary on our iridis (SSH). I am having a problem while trying to run (roary *.gff) on command line. The error looks something like that
EXCEPTION: Bio::Root::Exception -------------
MSG: Could not open PROKKA_SG1.gff.proteome.faa.intermediate.extracted.fa: No such file or directory
STACK: Error::throw
STACK: Bio::Root::Root::throw /local/software/perl-modules/share/perl5/Bio/Root/Root.pm:486
STACK: Bio::Root::IO::_initialize_io /local/software/perl-modules/share/perl5/Bio/Root/IO.pm:351
STACK: Bio::SeqIO::_initialize /local/software/perl-modules/share/perl5/Bio/SeqIO.pm:491
STACK: Bio::SeqIO::fasta::_initialize /local/software/perl-modules/share/perl5/Bio/SeqIO/fasta.pm:87
STACK: Bio::SeqIO::new /local/software/perl-modules/share/perl5/Bio/SeqIO.pm:372
STACK: Bio::SeqIO::new /local/software/perl-modules/share/perl5/Bio/SeqIO.pm:413
STACK: Bio::Roary::ExtractProteomeFromGFF::_fastatranslate /local/software/roary/3.0.3/source/sanger-pathogens-Roary-5685c8b/lib/Bio/Roary/ExtractProteomeFromGFF.pm:138
STACK: Bio::Roary::ExtractProteomeFromGFF::_convert_nucleotide_to_protein /local/software/roary/3.0.3/source/sanger-pathogens-Roary-5685c8b/lib/Bio/Roary/ExtractProteomeFromGFF.pm:152
STACK: Bio::Roary::ExtractProteomeFromGFF::fasta_file /local/software/roary/3.0.3/source/sanger-pathogens-Roary-5685c8b/lib/Bio/Roary/ExtractProteomeFromGFF.pm:43
STACK: Bio::Roary::CommandLine::ExtractProteomeFromGff::run /local/software/roary/3.0.3/source/sanger-pathogens-Roary-5685c8b/lib/Bio/Roary/CommandLine/ExtractProteomeFromGff.pm:79
STACK: /local/software/roary/3.0.3/source/sanger-pathogens-Roary-5685c8b/bin/extract_proteome_from_gff:18
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