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gtf file not found #263

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zhouyiqi91 opened this issue Sep 4, 2023 · 0 comments
Closed

gtf file not found #263

zhouyiqi91 opened this issue Sep 4, 2023 · 0 comments
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bug Something isn't working

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@zhouyiqi91
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Describe the bug
After running celescope snp mkref after celescope rna mkref, the gtf line in the celescope_genome.config file will be deleted. The deletion of the gtf line will cause error when celescope trys to located the gtf file, such as in the featureCounts step.

To Reproduce
Steps to reproduce the behavior:

  1. run celescope rna mkref
  2. run celescope snp mkref
  3. run celescope rna featureCounts

How to fix
Open the celescope_genome.config file under the genomeDir.
Add the gtf line:
gtf = {the file name of the gtf file} # for example, Homo_sapiens.GRCh38.99.filtered.gtf
Save and close the config file.

Version
1.17.0

@zhouyiqi91 zhouyiqi91 added the bug Something isn't working label Sep 4, 2023
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