Line profiler’s purpose is to evaluate datasets in a biological/biophysical context. The software recognizes line shaped structures in image data and computes their mean position and orientation with sub-pixel accuracy. For each line a mean intensity profile is calculated. Utilising the whole image the software prevents biases, caused by preselecting data subsets.
A detailed explanation and documentation can be found here.
An executable can be found on zenodo.
If this doesn't work for you:
- Clone git repository
- Open cmd and cd to repository
- Install requirements with:
pip install -r requirements.txt
- cd to src
- Run Line Profiler with:
python main.py
(1) Nobuyuki Otsu: A threshold selection method from grey level histograms. In: IEEE Transactions on Systems, Man, and Cybernetics. New York, 9.1979, S. 62–66. ISSN 1083-4419
(2) Felzenszwalb, Pedro F. and Huttenlocher, Daniel P. Distance Transforms of Sampled Functions, TR2004-1963, TR2004-1963 (2004)
(3) JOHN CANNY,A Computational Approach to Edge Detection, Editor(s): Martin A. Fischler, Oscar Firschein, Readings in Computer Vision, Morgan Kaufmann, 1987, Pages 184-203, ISBN 9780080515816, https://doi.org/10.1016/B978-0-08-051581-6.50024-6.