In addition to the nept dependencies, this project also requires pandas, matplotlib, seaborn, jupyter and svgutils libraries.
` conda install pandas, matplotlib seaborn jupyter svgutils `
- Pull emi_biconditional.
- Get MedPC data from the server. Put data files in emi_biconditional\cache\data\myproject folder.
This code contains analyses for:
- Behavior (medpc and cutom vdmlab operant box)
Which analyzes and plots the nosepoke durations, number of entries, latency to first entry, and percent of trials with responses.
The main objective of this series of tasks is to establish a procedure compatible with recording that involves the presentation of a discrete cue whose meaning is modulated by a seperate discrete cue.
Negative occasion setting
hM4Di DREADD in vHC
- 5 rats total; 2 DREADD experimental, 2 mCherry-alone controls, 1 WT CNO control
- 5 second feature cue (steady light)
- 5 second delay gap between feature and target
- 5 second target cue (pure tone ~85dB)
- Phase 1: 16 trials per session. 1:1 ratio; 8 rewarded trials, 8 unrewarded trials. Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Phase 2: 16 trials per session. 3:1 ratio; 4 rewarded trials, 12 unrewarded trials. Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Phase 3: 64 trials per session. 3:1 ratio; 16 rewarded trials, 48 unrewarded trials. Average 2 minute intertrial interval
Negative occasion setting
Ibotenic acid lesion of vHC
- 8 rats total; 2 lesion experimental, 2 sham controls, 2 WT controls
- 5 second feature cue (steady light)
- 5 second delay gap between feature and target
- 5 second target cue (pure tone ~85dB)
- 32 trials per session
- 3:1 ratio; 8 rewarded trials, 24 unrewarded trials
- Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Missing data from session 2 (data not saved properly from MedPC system)
Biconditional discrimination
Two daily sessions
- 8 rats total
- 10 second feature cue (steady or flashing light)
- 5 second delay gap between feature and target
- 10 second target cue (pure tone or white noise ~85dB)
- 32 trials per session
- 8 of each trial type, with a total of 16 rewarded and 16 unrewarded trials
- Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Counterbalanced
- Rats 5 and 8 were additionally run in the prototype vdmlab opperant box
Biconditional discrimination
Trial blocks
- 8 rats total
- 10 second feature cue (steady or flashing light)
- 1 second delay gap between feature and target
- 10 second target cue (pure tone or click ~85dB)
- 32 trials per session
- 8 of each trial type, with a total of 16 rewarded and 16 unrewarded trials
- Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Counterbalanced
- Trial sequence in blocks of 4 trials
Biconditional discrimination
Feature blocks
- 8 rats total
- 10 second feature cue (steady or flashing light)
- 1 second delay gap between feature and target
- 10 second target cue (pure tone or click ~85dB)
- 32 trials per session
- 8 of each trial type, with a total of 16 rewarded and 16 unrewarded trials
- Average 4 minute intertrial interval (range from 2.5 minuts to 5.5 minutes)
- Counterbalanced
- Both female and male rats
- Trial sequence in blocks of features
Occasion setting & biconditional discrimination
Long features
- 8 rats total
- Average 3.5 minute feature cue (steady or flashing light; range from 2.5 minuts to 4.5 minutes)
- No delay gap between feature and target
- 10 second target cue(s) (pure tone and white noise ~85dB introduced for the biconditional discrimination)
- 28 or 30 trials per session
- Equal ratios (1:1 for occasion setting; 1:1:1:1 for biconditional)
- 1 minute intertrial interval
- Counterbalanced
- Both female and male rats
- Phase 1: Conditioning tone only occasion setting (16 sessions)
- Phase 2: Counterconditioning (13 sessions)
- Phase 3: Re-training tone only occasion setting (16 sessions)
- Phase 4: Biconditional discrimination (16 sessions)
- Phase 5: Re-training tone only occasion setting (10 sessions)
- Phase 6: Alternating tone only and white noise only occasion setting sessions (15 noise sessions; 10 tone sessions)
- Phase 7: Joint tone and noise sessions (blocks of noise or tone for 16 sessions)