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additional files in G3_TOY/data #2
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Did you eventually figure out what those input files are? @kelsi-kw I tried to blast the sequence and some of these blast to both CCR2 and CCR5, but I'm not sure how the authors pick those sequences. My guess is that the mispriming file is prob the sequence similar to the guide but I wonder how do they find it. Also, do you know what is the ots.bed file and how was it generated? Maybe we should reach out to the authors. |
I did end up reaching out to them! |
Thank you so much for sharing the info, @kelsi-kw !! This is very useful :D |
Also, @kelsi-kw do you know what is the ots.bed file? From the name it looks like "off-target site", but I'm not sure how to generate it. |
The only thing I found for that is "--onTarget name of ON-target bed file (default "ots.bed")". |
Interesting, I look at the codes on their G3_TOY/data page (https://github.com/AG-Boerries/CAST-Seq/blob/master/samples/G3_TOY/data/ots.bed) and ots.bed is "chr3 46372985 46373015 G3_OTS 1000 +". I wonder how they generate it, especially the column saying "G3_OTS 1000". |
The region and naming is following BED file format.
https://en.wikipedia.org/wiki/BED_(file_format)
…On Fri, May 19, 2023 at 11:41 AM peggy314pch ***@***.***> wrote:
Interesting, I look at the codes on their G3_TOY/data page (
https://github.com/AG-Boerries/CAST-Seq/blob/master/samples/G3_TOY/data/ots.bed)
and ots.bed is "chr3 46372985 46373015 G3_OTS 1000 +". I wonder how they
generate it, especially the column saying "G3_OTS 1000".
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Hi, have you figured out the meaning of "1000", I'm a new user of this pipeline and I'm also want to konw what the 1000 means? |
As far as I can tell:
I recommend using Snapgene for this, very helpful. |
Hello! I've been able to get Cast-seq to run on the G3_TOY data, but I was curious what the other files are that are called on from the G3_TOY directory. I can't find a description in the paper or in the repo. I am trying to figure out from my data, what these files should be.
headTOhead.fa
linker.fa
linker_RC.fa
mispriming.fa
neg.fa
pos.fa
Thank you for your help!
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