Support for non mapped accession with taxid

requirements.txt

@@ -1,4 +1,5 @@
 ftputil
 peewee==2.8.1
+psycopg2
 PyMySQL
 nose

taxadb/accession.py

@@ -2,10 +2,11 @@
 # -*- coding: utf-8 -*-
 
 from taxadb.schema import *
+import sys
 
 
 def taxid(acc_number_list, db_name, table):
-    """given a list of acession numbers, yield
+    """given a list of accession numbers, yield
     the accession number and their associated taxids as tuples
 
     Arguments:
@@ -16,11 +17,14 @@ def taxid(acc_number_list, db_name, table):
     database = pw.SqliteDatabase(db_name)
     db.initialize(database)
     db.connect()
+    _check_table_exists(table)
     with db.atomic():
         query = table.select().where(table.accession << acc_number_list)
-        # taxid = table.get(table.accession == acc_number)
         for i in query:
-            yield (i.accession, i.taxid.ncbi_taxid)
+            try:
+                yield (i.accession, i.taxid.ncbi_taxid)
+            except Taxa.DoesNotExist:
+                _unmapped_taxid(i.accession)
     db.close()
 
 
@@ -36,11 +40,14 @@ def sci_name(acc_number_list, db_name, table):
     database = pw.SqliteDatabase(db_name)
     db.initialize(database)
     db.connect()
+    _check_table_exists(table)
     with db.atomic():
         query = table.select().where(table.accession << acc_number_list)
-        # taxid = table.get(table.accession == acc_number)
         for i in query:
-            yield (i.accession, i.taxid.tax_name)
+            try:
+                yield (i.accession, i.taxid.tax_name)
+            except Taxa.DoesNotExist:
+                _unmapped_taxid(i.accession)
     db.close()
 
 
@@ -56,21 +63,25 @@ def lineage_id(acc_number_list, db_name, table):
     database = pw.SqliteDatabase(db_name)
     db.initialize(database)
     db.connect()
+    _check_table_exists(table)
     with db.atomic():
         query = table.select().where(table.accession << acc_number_list)
         for i in query:
-            lineage_list = []
-            current_lineage = i.taxid.tax_name
-            current_lineage_id = i.taxid.ncbi_taxid
-            parent = i.taxid.parent_taxid
-            while current_lineage != 'root':
-                lineage_list.append(current_lineage_id)
-                new_query = Taxa.get(Taxa.ncbi_taxid == parent)
-
-                current_lineage = new_query.tax_name
-                current_lineage_id = new_query.ncbi_taxid
-                parent = new_query.parent_taxid
-            yield (i.accession, lineage_list)
+            try:
+                lineage_list = []
+                current_lineage = i.taxid.tax_name
+                current_lineage_id = i.taxid.ncbi_taxid
+                parent = i.taxid.parent_taxid
+                while current_lineage != 'root':
+                    lineage_list.append(current_lineage_id)
+                    new_query = Taxa.get(Taxa.ncbi_taxid == parent)
+
+                    current_lineage = new_query.tax_name
+                    current_lineage_id = new_query.ncbi_taxid
+                    parent = new_query.parent_taxid
+                yield (i.accession, lineage_list)
+            except Taxa.DoesNotExist:
+                _unmapped_taxid(i.accession)
     db.close()
 
 
@@ -86,17 +97,51 @@ def lineage_name(acc_number_list, db_name, table):
     database = pw.SqliteDatabase(db_name)
     db.initialize(database)
     db.connect()
+    _check_table_exists(table)
     with db.atomic():
         query = table.select().where(table.accession << acc_number_list)
         for i in query:
-            lineage_list = []
-            current_lineage = i.taxid.tax_name
-            parent = i.taxid.parent_taxid
-            while current_lineage != 'root':
-                print(current_lineage)
-                lineage_list.append(current_lineage)
-                new_query = Taxa.get(Taxa.ncbi_taxid == parent)
-                current_lineage = new_query.tax_name
-                parent = new_query.parent_taxid
-            yield (i.accession, lineage_list)
+            try:
+                lineage_list = []
+                current_lineage = i.taxid.tax_name
+                parent = i.taxid.parent_taxid
+                while current_lineage != 'root':
+                    print(current_lineage)
+                    lineage_list.append(current_lineage)
+                    new_query = Taxa.get(Taxa.ncbi_taxid == parent)
+                    current_lineage = new_query.tax_name
+                    parent = new_query.parent_taxid
+                yield (i.accession, lineage_list)
+            except Taxa.DoesNotExist:
+                _unmapped_taxid(i.accession)
     db.close()
+
+
+def _check_table_exists(table):
+    """Check a table exists in the database
+
+    Arguments:
+    table -- table name
+    Throws `SystemExit` if table does not exist
+    """
+    if not table.table_exists():
+        print("Table %s does not exist" % (str(table._meta.db_table)), file=sys.stderr)
+        sys.exit(1)
+    return True
+
+def _unmapped_taxid(acc, exit=False):
+    """Prints an error message on stderr an accession number is not mapped with a taxid
+
+    Source ftp://ftp.ncbi.nlm.nih.gov/pub/taxonomy/accession2taxid/README
+    >> If for some reason the source organism cannot be mapped to the taxonomy database,
+    the column will contain 0.<<
+
+    Arguments:
+    acc -- Accession number not mapped with taxid
+    exit -- Exit with code 1, default False
+    """
+    print("No taxid mapped for accession %s" % str(acc), file=sys.stderr)
+    if exit:
+        sys.exit(1)
+    return True
+

taxadb/app.py

@@ -4,17 +4,13 @@
 import peewee as pw
 
 import os
-import gzip
 import tarfile
 import ftputil
 import argparse
 
 from taxadb import util
 from taxadb import parse
 
-from taxadb import accession
-from taxadb import taxid
-
 from taxadb.schema import *
 
 
@@ -87,6 +83,15 @@ def create_db(args):
             user=args.username,
             password=args.password
             )
+    elif args.dbtype == 'postgres':
+        if args.username is None or args.password is None:
+            print('--dbtype postgres requires --username and --password.\n')
+        database = pw.PostgresqlDatabase(
+            args.dbname,
+            user=args.username,
+            password=args.password
+            )
+
     div = args.division  # am lazy at typing
     db.initialize(database)
 
@@ -120,16 +125,19 @@ def create_db(args):
     )
     # insert in database
     with db.atomic():
-        for i in range(0, len(taxa_info_list), 500):
-            Taxa.insert_many(taxa_info_list[i:i+500]).execute()
+        for i in range(0, len(taxa_info_list), args.chunk):
+            Taxa.insert_many(taxa_info_list[i:i+args.chunk]).execute()
     print('Taxa: completed')
 
     with db.atomic():
         for table, acc_file in acc_dl_dict.items():
             for data_dict in parse.accession2taxid(
-                    args.input + '/' + acc_file):
-                table.create(**data_dict)
+                    args.input + '/' + acc_file, args.chunk):
+                table.insert_many(data_dict[0:args.chunk]).execute()
             print('%s: %s added to database' % (table, acc_file))
+            print('Creating index for field accession ... ', end="")
+            db.create_index(table, ['accession'], unique=True)
+            print('created.')
     print('Sequence: completed')
     db.close()
 
@@ -171,6 +179,14 @@ def main():
         description='build the database',
         help='build the database'
     )
+    parser_create.add_argument(
+        '--chunk',
+        '-c',
+        metavar='<#chunk>',
+        type=int,
+        help='Number of sequences to insert in bulk',
+        default=500
+    )
     parser_create.add_argument(
         '--input',
         '-i',
@@ -188,9 +204,9 @@ def main():
     parser_create.add_argument(
         '--dbtype',
         '-t',
-        choices=['sqlite', 'mysql'],
+        choices=['sqlite', 'mysql', 'postgres'],
         default='sqlite',
-        metavar='[sqlite|mysql]',
+        metavar='[sqlite|mysql|postgres]',
         help='type of the database (default: %(default)s))'
     )
     parser_create.add_argument(
@@ -204,12 +220,12 @@ def main():
     parser_create.add_argument(
         '--username',
         '-u',
-        help='Username to login as (required for MySQLdatabase)'
+        help='Username to login as (required for MySQLdatabase and PostgreSQLdatabase)'
     )
     parser_create.add_argument(
         '--password',
         '-p',
-        help='Password to use (required for MySQLdatabase)'
+        help='Password to use (required for MySQLdatabase and PostgreSQLdatabase)'
     )
     parser_create.set_defaults(func=create_db)
 

taxadb/parse.py

@@ -2,7 +2,7 @@
 # -*- coding: utf-8 -*-
 
 import gzip
-
+from taxadb.schema import Taxa
 
 def taxdump(nodes_file, names_file):
     """Parse the nodes.dmp and names.dmp files (from taxdump.tgz) and insert
@@ -41,27 +41,65 @@ def taxdump(nodes_file, names_file):
 
     # merge the two dictionaries
     taxa_info_list = list()
-    taxa_info = {}
     for nodes, names in zip(nodes_data, names_data):
         taxa_info = {**nodes, **names}  # PEP 448, requires python 3.5
         taxa_info_list.append(taxa_info)
     print('merge successful')
     return taxa_info_list
 
 
-def accession2taxid(acc2taxid):
-    """Parse the accession2taxid files. and insert
-    squences in the Sequence table.
+#def accession2taxid(acc2taxid):
+#    """Parse the accession2taxid files. and insert
+#    squences in the Sequence table.
+#
+#    Arguments:
+#    acc2taxid -- input file (gzipped)
+#    """
+#    with gzip.open(acc2taxid, 'rb') as f:
+#        f.readline()  # discard the header
+#        for line in f:
+#            line_list = line.decode().rstrip('\n').split('\t')
+#            data_dict = {
+#                'accession': line_list[0],
+#                'taxid': line_list[2]
+#            }
+#            yield(data_dict)
+
+
+def accession2taxid(acc2taxid, chunk):
+    """Parses the accession2taxid files and insert sequences in Sequences table(s).
 
     Arguments:
     acc2taxid -- input file (gzipped)
+    chunk -- Chunk size of entries to gather before yielding, default 500
     """
+    # Some accessions (e.g.: AAA22826) have a taxid = 0
+    entries = []
+    counter = 0
+    taxids = {}
+    if not chunk:
+        chunk = 500
     with gzip.open(acc2taxid, 'rb') as f:
         f.readline()  # discard the header
         for line in f:
             line_list = line.decode().rstrip('\n').split('\t')
-            data_dict = {
-                'accession': line_list[0],
-                'taxid': line_list[2]
-            }
-            yield(data_dict)
+            if not line_list[2] in taxids:
+                try:
+                    Taxa.get(Taxa.ncbi_taxid == int(line_list[2]))
+                    taxids[line_list[2]] = True
+                except Taxa.DoesNotExist:
+                    taxids[line_list[2]] = False
+                    continue
+            if taxids[line_list[2]]:
+                data_dict = {
+                    'accession': line_list[0],
+                    'taxid': line_list[2]
+                }
+                entries.append(data_dict)
+                counter += 1
+            if counter == chunk:
+                yield(entries)
+                entries = []
+                counter = 0
+        if len(entries):
+            yield(entries)