Skip to content

Snakemake pipeline for structural variant calling from Nanopore long reads using NGLMR for mapping and Sniffles for variant calling.

Notifications You must be signed in to change notification settings

JamieCFreeman/nglmr_SV

Folders and files

NameName
Last commit message
Last commit date

Latest commit

 

History

10 Commits
 
 
 
 
 
 
 
 
 
 

Repository files navigation

nglmr_SV

Using Nanopore reads for variant calling.

Largely based on https://github.com/wdecoster/nano-snakemake and https://github.com/nanoporetech/ont_tutorial_sv

  1. Map reads with NGLMR.
  2. Call SV with Sniffles (min read support set low (3) for my low coverage data).
  3. Use Survivor to merge per sample VCFs (paramters for when to merge variants are important).
  4. Force Sniffles to call genotype at the site of each variant in the merged file.

Conda is used to manage environments on a per rule basis, so be sure to deploy Snakemake with the --use-conda flag.

DAG

About

Snakemake pipeline for structural variant calling from Nanopore long reads using NGLMR for mapping and Sniffles for variant calling.

Resources

Stars

Watchers

Forks

Releases

No releases published

Packages

No packages published