This repository contains information of a training I facilitated together with Dr. Ngara Mtakai and Kennedy Mwangi. It is an easy to follow write-up to understand RNA-Seq pipeline

The following sections are covered in this tutorial;

• Bioinformatics background and resources
• Next-generation sequencing (NGS) concepts and applications
• Gene expression profiling using RNA-sequencing technique
• Basic command line for unix/linux users
• Conda environment installation and basics
• NGS datasets
• Read trimming
• Quality control (QC) analysis
• Reference mapping
• Mapping quality evaluation
• Gene expression quantification
• Differential expression analysis
• Basic visualisation