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AMF communities under simulated short-term drought in a long-term farming experiment with organic and conventional management practices.

• we examined 48 samples, 24 samples per time point
  
• single-molecule real-time (SMRT) DNA sequencing was used, 1.5kb long fragment (part of SSU and LSU, and entire ITS region) was amplified
  
• 4 Sequel SMRT cells were sequenced on PacBio Sequel platform (samples were prepared with SMRTbell™ Template Prep Kit 1.0-SPv3)
  
• circular consensus sequencing (CCS reads), in total 481165 were generated; 2500 to 19000 reads per sample
  
• demultiplexing with Lima (PacBio barcode demultiplexer, https://github.com/pacificbiosciences/barcoding)
  
• as next DADA2, version 1.12.1. in R version 3.6.1
  
• UNITE database (https://unite.ut.ee/), v8.2 used for taxonomic classification
• The raw sequencing data (fastq format) is publically available at the EMBL-ENA database (http://www.ebi.ac.uk) under project ID PRJEB38629