IMAO

Imaris-based Morphological Analysis of Osteocytes (IMAO) is a workflow to estimate the morphology of osteocytes thoroughly and comprehensively.

00. Project Structure

IMAO
- 01.Analysis.R                                                         # File to run
- Functions.R                                                          # Essential functions
-- data\                                                                    # Folder contains Imaris results
--- Sample1_FilamentsNucleusCut_Statistics        # Imaris-produced results of filaments without nucleus part
--- Sample1_FilamentsNucleusFill_Statistics         # Imaris-produced results of filaments with nucleus as filaments start point
--- Sample1_Surface_Statistics                              # Imaris-produced morphological results of nucleus
--- Sample1.ims.hoc                                                # Imaris-produced structure file of filaments with nucleus as filaments start point
--- ...
--- SampleN_FilamentsNucleusCut_Statistics
--- SampleN_FilamentsNucleusFill_Statistics
--- SampleN_Surface_Statistics
--- SampleN.ims.hoc
-- results\                                                                 # Results folder
--- Osteocytes.Processes.Analysis.Summary         # The results of each osteocyte, each sample, and Moran's index.