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mutscan
when use fastp + gencore , can not use GATK SamToFastq. gencore copy the flag of R1 reads as R2 reads (or R2 as R1)? For example, Before gencore
fastp
gencore
GATK SamToFastq
A00877:990:HL5F3DSX3:4:2111:6361:29731:UMI_CCCTGAGTGT 1187 chr1 27023101 60 2S111M = 27023101 111 GCGGGAAAGGAGCTGCAGGACGGGGCCGAGAGCAATGGGGGTGGCGGCGGCGGCGGAGCCGGCAGCGGCGGCGGGCCCGGCGCGGAGCCGGACCTGAAGAACTCGAACGGGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFF: NM:i:0 MD:Z:111 MC:Z:2S111M AS:i:111 XS:i:20 RG:Z:M30713-3MJ15_M30713-3MJ15_llluma_L4 A00877:990:HL5F3DSX3:4:2111:6361:29731:UMI_CCCTGAGTGT 1107 chr1 27023101 60 2S111M = 27023101 -111 GCGGGAAAGGAGCTGCAGGACGGGGCCGAGAGCAATGGGGGTGGCGGCGGCGGCGGAGCCGGCAGCGGCGGCGGGCCCGGCGCGGAGCCGGACCTGAAGAACTCGAACGGGAA FFFFFF,FFFFF::FFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:111 MC:Z:2S111M AS:i:111 XS:i:20 RG:Z:M30713-3MJ15_M30713-3MJ15_llluma_L4
After gencore
A00877:990:HL5F3DSX3:4:2111:6361:29731:UMI_CCCTGAGTGT 1187 chr1 27023101 60 2S111M = 27023098 111 GCGGGAAAGGAGCTGCAGGACGGGGCCGAGAGCAATGGGGGTGGCGGCGGCGGCGGAGCCGGCAGCGGCGGCGGGCCCGGCGCGGAGCCGGACCTGAAGAACTCGAACGGGAA FFFFFFFFFFFFF:FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF NM:i:0 MD:Z:12G2G17T24G8G11G1G18T1G8 MC:Z:3M1D110M AS:i:66 XS:i:22 RG:Z:M30713-3MJ15_M30713-3MJ15_llluma_L4 FR:i:3 A00877:990:HL5F3DSX3:4:2111:6361:29731:UMI_CCCTGAGTGT 1187 chr1 27023101 60 2S111M = 27023101 111 GCGGGAAAGGAGCTGCAGGACGGGGCCGAGAGCAATGGGGGTGGCGGCGGCGGCGGAGCCGGCAGCGGCGGCGGGCCCGGCGCGGAGCCGGACCTGAAGAACTCGAACGGGAA FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF: NM:i:0 MD:Z:111 MC:Z:2S111M AS:i:111 XS:i:20 RG:Z:M30713-3MJ15_M30713-3MJ15_llluma_L4 FR:i:3
Am I making a mistake, or is there some other solution。 I want run fastp with the ConsensusReads. Thank you for your great work!
The text was updated successfully, but these errors were encountered:
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when use
fastp
+gencore
, can not useGATK SamToFastq
.gencore
copy the flag of R1 reads as R2 reads (or R2 as R1)?For example,
Before
gencore
After
gencore
Am I making a mistake, or is there some other solution。
I want run
fastp
with the ConsensusReads.Thank you for your great work!
The text was updated successfully, but these errors were encountered: