GatingSet2flowJo without transformation

[mikejiang]

flowJo expect the gate coordinates to be at raw scale. So transformation is required to be present in GatingSet in order to inverse transform the gates.
You can do the compensation and transformation directly to GatingSet instead of FCS so that they will be all recorded in GatingSet. (See vignette )

There is flowWorkspace::asinhtGml2_trans constructor you can use if you prefer to asinh

On 07/12/2016 07:25 AM, Brian Muchmore wrote:

Hi Mike,

So, I run the following code

flowjofile <- file.path
GatingSet2flowJo(gs, flowjofile)

And get the following error

Error: No transformation is found in GatingSet!

I run an asinh transformation on my original FCS files and then rewrite them as new FCS files before running openCyto. Is it possible to augment the GatingSet2flowJo function to ignore the transformation information (or lack thereof)?

Thanks.

-Brian