This repository contains updated plant and arthropod metabarcoding reference databases produced using the MetaCurator pipeline (https://github.com/RTRichar/MetaCurator). The databases represent considerable upgrades relative to prior works (https://doi.org/10.1111/mec.14975 and https://doi.org/10.7717/peerj.5126) and details on their production and contents can be found at https://www.biorxiv.org/content/10.1101/672782v1. Refer to the 'Workflow.md' file of this project to find the commands used during curation.
This archive also contains the files and commands used to test the sensitivity of MetaCurator to variation in the seed representative sequences used to initiate curation, the HMMER E-value cutoff used and the minimun proportional HMM profile length coverage required during curation. These can be found in the ParameterSensitivityAnalysis.zip file.