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Hi,
When i running the step of PASCALL,I saw the ployaDB in optional.So So I didn't choose that option.Now i have a problem that I have a huge amount of data to sift through right now (with 70,000 PASs).How can i use the polyaDB to filter the data via myself instead of running PAScall.
The text was updated successfully, but these errors were encountered:
Hi,
In the PAScall step, SCAPTURE will generate three BED files for exonic, intronic, 3primeExtended poly(A) sites, which were named as "Your_Output_Prefix.*.peaks.annotated.bed".
SCAPTURE could solely run the "evaluate process" with files mentioned above and polyADB hg38 file with comands below:
We take exonic poly(A) sites BED file as an example here, which could be replaced by "intronic.peaks.annotated.bed" and "3primeExtended.peaks.annotated.bed" files.
Then, the output of "scapture_evaluate" step will be "Your_Output_Prefix.exonic.peaks.evaluated.bed", and could directly be applied to the downstream PASmerge or PASquant step.
Hi,
When i running the step of PASCALL,I saw the ployaDB in optional.So So I didn't choose that option.Now i have a problem that I have a huge amount of data to sift through right now (with 70,000 PASs).How can i use the polyaDB to filter the data via myself instead of running PAScall.
The text was updated successfully, but these errors were encountered: