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Added Sequence method to Feature object #26

Merged
merged 16 commits into from
Jul 17, 2020
Merged

Added Sequence method to Feature object #26

merged 16 commits into from
Jul 17, 2020

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Koeng101
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@Koeng101 Koeng101 commented Jul 3, 2020

This pull request adds a minimally viable way to get the sequence of features. This can be used to fill codon tables for optimization, or to just get the DNA sequence of features.

Testing is currently blocked with bug #24 .

Definitely needs some improvement, but as-is it works for the following from this page (basically, all the Location types that are relevant for CDSs): http://www.insdc.org/files/feature_table.html#3.4.3

340..565
join(12..78,134..202)
complement(34..126)
complement(join(2691..4571,4918..5163))
join(complement(4918..5163),complement(2691..4571))

Usage:

f := feature.Sequence(annotatedSequence)

Keoni Gandall and others added 14 commits July 3, 2020 07:41
Adds a method to Feature known as Sequence. This was added
anticipating the codon optimization feature in #11, which
will require parsing codons from features to build codon tables.

Additionally, added manually edited test file t4_intron and fixed
one bug in io.go.
This commit adds degenerate bases and makes a public method for
ReverseComplement, as the full functionality for reverse complementation
was previously split in the getSeq function and reverseComplement,
which has been renamed to reverseBase.

Degenerate base pairs were added using the table from
http://arep.med.harvard.edu/labgc/adnan/projects/Utilities/revcomp.html
.
Removed most single or double letter variable names and replaced with
more human reable names.
@TimothyStiles
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@Koeng101 please review then I'll review again and merge!

@TimothyStiles TimothyStiles merged commit 6264d61 into bebop:prime Jul 17, 2020
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2 participants