Whole genome sgRNA design database for human and mouse. Due to the limites of file size, the bam files are uploaded to http://enhancer.sdsc.edu/CREST-web/
We developed an exhaustive search algorithm(CREST-web) to calculate all off-targets accurately. CREST-web calculates genome-wide off-target candidates in 7 hours using 8 CPUs. And we introduced a new scoring function to evaluate the off-targets effects considering both of the number and the positions of the mismatches. The outputs in standard SAM/BAM format.
Diao, Y., Fang, R., Li, B., Meng, Z., Yu, J., Qiu, Y., Lin, K. C., Huang, H., Liu, T., Marina, R. J., Jung, I., Shen, Y., Guan, K.-L., and Ren, B. A tiling- deletion based genetic screen for cis-regulatory element identification in mammalian cells. Nature Methods.