No tips error trying to classify

[christineolson-ucsf]

Hello, in trying to use Greengenes2 with a FeatureData[Sequence] object, I get the following error saying "no requested tips found", full error message also attached. Can you please help me resolve this?
![image](https://user-images.githubusercontent.com/91097042/

219550993-ba1f3b3e-fbae-47ab-b22a-b7ab5a86e2d1.png)

[christineolson-ucsf]

Other image of command run and initial error:

[wasade]

Thanks @christineolson-ucsf! Could you tell me how the FeatureData[Sequence] artifact was constructed, and are these 16S V4?

I am very interested in improving the error messages the plugin produces, so I'm hoping to use this github issue to guide a data detection method to better inform users.

[Ilaria-Pirona]

I am having the same problem while running qiime greengenes2 taxonomy-from-table (qiime2-2022.8). I am trying to assing the taxonomy to my V3-V4 regions. I followed the tutorial at link and the first command of the section "If you have V4 data" did not raise errors.

[wasade]

Hi @Ilaria-Pirona,

What specific primers were used? The majority of the data placed with DEPP are derived from 515F-806R. If your primers are relative to other positions, then it is unlikely your ASVs will be represented. In that scenario, I recommend using the non-v4-16s action

[ebadiea]

I got the same error "No requested tips found" and don't know how to fix it

[wasade]

Thanks, @ebadiea. Can you describe the data, and the commands used?

[wasade]

Please reopen if this is still a problem