- Added a new module called NanoPreprocessSimple that starts from fastq files instead of fast5 files. It allows the analysis of multiple files at a time.
- Added support to vbz compressed fast5 https://github.com/nanoporetech/vbz_compression in NanoPreprocess, NanoMod and NanoTail
- NanoPreprocess now outputs also CRAM files and can do downsampling with the parameter --downsampling
- NanoPreprocess allows performing variant calling using medaka (BETA)
- NanoPreprocess allows performing demultiplexing with GUPPY
- Added plots for Epinano output in NanoMod
- Added a conversion of Tombo results in bed format in NanoMod
- Added a INSTALL.sh file for automatically retrieve guppy 3.4.5 from https://mirror.oxfordnanoportal.com/, place it in NanoPreprocess/bin and making the required links
- Added profiles for being used locally and on the CRG SGE cluster
This is the original version published in the paper MasterOfPores: A Workflow for the Analysis of Oxford Nanopore Direct RNA Sequencing Datasets