KnowEnG's Gene Set Characterization Pipeline

This is the Knowledge Engine for Genomics (KnowEnG), an NIH BD2K Center of Excellence, Gene Set Characterization Pipeline.

This pipeline ranks a user supplied gene set against a KnowEnG's gene sets collection.

There are three gene set characterization methods that one can choose from:

Options	Method	Parameters
Fisher exact test	Fisher	fisher
Discriminative Random Walks with Restart	DRaWR	DRaWR
Net Path	Net Path	net_path

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How to run this pipeline with Our data

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1. Clone the GeneSet_Characterization_Pipeline Repo

 git clone https://github.com/KnowEnG/GeneSet_Characterization_Pipeline.git

2. Install the following (Ubuntu or Linux)

apt-get install -y python3-pip
apt-get install -y libblas-dev liblapack-dev libatlas-base-dev gfortran
pip3 install numpy==1.11.1
pip3 install pandas==0.18.1
pip3 install scipy==0.19.1
pip3 install scikit-learn==0.17.1
apt-get install -y libfreetype6-dev libxft-dev
pip3 install matplotlib==1.4.2
pip3 install pyyaml
pip3 install knpackage

3. Change directory to GeneSet_Characterization_Pipeline

cd GeneSet_Characterization_Pipeline

4. Change directory to test

cd test

5. Create a local directory "run_dir" and place all the run files in it

make env_setup

6. Select and run a gene set characterization option:

• Run fisher pipeline
  

make run_fisher

• Run DRaWR pipeline
  

make run_drawr

• Run Net Path pipeline
  

make run_netpath

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How to run this pipeline with Your data

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Follow steps 1-3 above then do the following:

* Create your run directory

mkdir run_directory

* Change directory to the run_directory

cd run_directory

* Create your results directory

mkdir results_directory

* Create run_paramters file (YAML Format)

Look for examples of run_parameters in the GeneSet_Characterization_Pipeline/data/run_files BENCHMARK_1_fisher.yml

* Run the GeneSet Characterization Pipeline:

• Update PYTHONPATH environment variable

export PYTHONPATH='./src':$PYTHONPATH    

• Run

python3 ../src/geneset_characterization.py -run_directory ./run_dir -run_file BENCHMARK_1_fisher.yml

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Description of "run_parameters" file

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Key	Value	Comments
method	DRaWR or fisher or net_path	Choose DRaWR or fisher or Net Path as the gene set characterization method
pg_network_name_full_path	directory+pg_network_name	Path and file name of the 4 col property file
gg_network_name_full_path	directory+gg_network_name	Path and file name of the 4 col network file(needed in DRaWR and Net Path)
spreadsheet_name_full_path	directory+spreadsheet_name	Path and file name of user supplied gene sets
gene_names_map	directory+gene_names_map	Map ENSEMBL names to user specified gene names
results_directory	directory	Directory to save the output files
rwr_max_iterations	500	Maximum number of iterations without convergence in random walk with restart(needed in DRaWR and Net Path)
rwr_convergence_tolerence	0.0001	Frobenius norm tolerence of spreadsheet vector in random walk(needed in DRaWR and Net Path)
rwr_restart_probability	0.5	alpha in V_(n+1) = alpha * N * Vn + (1-alpha) * Vo (needed in DRaWR and Net Path)
k_space	100	number of the new space dimensions in SVD(only needed in Net Path)

pg_network_name = kegg_pathway_property_gene.edge
gg_network_name = STRING_experimental_gene_gene.edge
spreadsheet_name = ProGENI_rwr20_STExp_GDSC_500.rname.gxc.tsv
gene_names_map = ProGENI_rwr20_STExp_GDSC_500_MAP.rname.gxc.tsv

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Description of Output files saved in results directory

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• Output files of all three methods save sorted properties for each gene set with name {method}_ranked_by_property_{timestamp}.df.
  

user gene set name1	user gene set name2	...	user gene set name n
property (most significant)	property (most significant)	...	property (most significant)
...	...	...	...
property (least significant)	property (least significant)	...	property (least significant)

• Fisher method saves one output file with seven columns and it is sorted in descending order based on pval. The name of the file is fisher_sorted_by_property_score_{timestamp}.df.

user_gene_set	property_gene_set	pval	universe_count	user_count	property_count	overlap_count
user gene 1	property 1	float	int	int	int	int
...	...	...	...	...	...	...
user gene n	property m	float	int	int	int	int

• DRaWR method saves two output files with five columns and they are sorted in descending order based on difference_score. The files are DRaWR_sorted_by_gene_score_{timestamp}.df and DRaWR_sorted_by_property_score_{timestamp}.df

user_gene_set	gene_node_id	difference_score	query_score	baseline_score
user gene 1	gene node 1	float	float	float
...	...	...	...	...
user gene n	gene node m	float	float	float

user_gene_set	property_gene_set	difference_score	query_score	baseline_score
user gene 1	property 1	float	float	float
...	...	...	...	...
user gene n	property m	float	float	float

• Net Path method saves one output file with three columns and it is sorted in descending order based on cosine_sum. The name of the file is net_path_sorted_by_property_score_{timestamp}.df.

user_gene_set	property_gene_set	cosine_sum
user gene 1	property 1	float
...	...	...
user gene n	property m	float