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MSMSScans TopNoverN plot shows equally high bars for all 'N' #119

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cbielow opened this issue Aug 23, 2022 · 6 comments
Closed

MSMSScans TopNoverN plot shows equally high bars for all 'N' #119

cbielow opened this issue Aug 23, 2022 · 6 comments

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@cbielow
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cbielow commented Aug 23, 2022

e.g.
image

The reason is that MaxQuant switched from +/- in the identified column to + and `` (empty) since MaxQuant 2.x...

@cbielow cbielow changed the title MSMSScans TOP-N plot shows equally high bars for all 'N' MSMSScans TopNoverN plot shows equally high bars for all 'N' Aug 23, 2022
@czanetti
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czanetti commented Sep 1, 2022

hello, I get the error:
Error in workerFcn(.self, df, ...) : unique(d_msmsScan$identified) %in% c("-", "+") non sono tutti TRUE (translation : "are not all true")

I installed the package with recent commits in this way

library(remotes)
install_github("cbielow/PTXQC",
               build_vignettes = TRUE, 
               dependencies = TRUE, ref = "r1.0.14")

MaxQuant Version 2.0.3.0

where do you think the issue could lie?

@cbielow
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cbielow commented Sep 1, 2022

Iäm a bit puzzled why this did not show up in my test data, but its pretty clear where the problem is.
A fix is coming, but will take some days, since currently on vacation :)

@czanetti
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czanetti commented Sep 5, 2022

I tried to fix the problem (my first PR 😇), it worked for me. #122
Thanks for your great work !

@cbielow
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cbielow commented Sep 6, 2022 via email

@cbielow
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cbielow commented Sep 11, 2022

FYI: the reason I did not catch this, is that my MQ2.x dataset was run without dependent peptides. Hence the failing metric did not get invoked :/
Thanks for the fix!

@cbielow
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cbielow commented Sep 21, 2022

fixed in #120

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