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Issues creating and detecting the the iguide environment #70
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Hi Pedro, Thanks for checking out iGUIDE, it's been a lot of fun to put together and I still hope to add some improvements on it. I'm having issues recreating your issue though. Could you check to see which version of conda you are running? From the verbose installation, it looks like you already had miniconda installed. I find it easiest to clone iGUIDE into my home directory and then setup up everything from there, rather than a temp directory. Seeing that miniconda is installed on your system at root level, you may need to get your sys admin to update if that is a problem, or you could have them run the install of iguide and see if that works out. I've retriggered the automated testing for iGUIDE through CircleCI and also ran it myself on the system used to develop the software, but I couldn't recreate your errors. Neither showed any issues with the installation. Could you let me know a bit more about what your system is like or your goals of running iGUIDE? Best, |
Hi Chris,
Thanks a lot for getting back to us. I'll try to clone it first and install
it from there. Also will double-check with our IT team to make sure that
there isn't any incompatibility related to that too. I'll test it ASAP and
will get back to you soon.
Again, thanks a lot for your support!
Have a great week ahead!!
Best,
Pedro.
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…On Mon, Jun 1, 2020 at 7:32 AM Chris Nobles ***@***.***> wrote:
Hi Pedro,
Thanks for checking out iGUIDE, it's been a lot of fun to put together and
I still hope to add some improvements on it.
I'm having issues recreating your issue though. Could you check to see
which version of conda you are running? From the verbose installation, it
looks like you already had miniconda installed.
I find it easiest to clone iGUIDE into my home directory and then setup up
everything from there, rather than a temp directory.
Seeing that miniconda is installed on your system at root level, you may
need to get your sys admin to update if that is a problem, or you could
have them run the install of iguide and see if that works out.
I've retriggered the automated testing for iGUIDE through CircleCI and
also ran it myself on the system used to develop the software, but I
couldn't recreate your errors. Neither showed any issues with the
installation.
Could you let me know a bit more about what your system is like or your
goals of running iGUIDE?
Best,
Chris
[image: image]
<https://user-images.githubusercontent.com/12237291/83419319-e6ee2000-a3f2-11ea-8109-215232314dd6.png>
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Hi Guy, Could you post the results from running the install script with the "-v" flag, this should help in debugging. Seems like there may be some conflict if you have both installed (miniconda and anaconda). If that is an issue, then we could likely just do the install in a more manual fashion, but run it through anaconda. A manual setup may be helpful if this turns out to be a reoccurring issue. Best, |
Alright, let's try an manual install. I should likely update the docs with this process as well. If it's not clear, anything in the code blocks should be input into the command line. My preferred installation has both the miniconda and iGUIDE directories in my home directory. Installing minicondaSkip to installing iGUIDE if you already have miniconda or anaconda installed. These can be executed in your home directory.
Installing iGUIDEThe following commands should be called from within the iGUIDE directory (/path/to/iGUIDE/) after the repository is cloned. (If you need help on this part, just let me know).
TestingTests to make sure the components were installed correctly.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true || echo false'
|
Hi Chris,
Thanks for providing the manual installation. I've tried to install in two
different OS:
- macos Catalina 10.15.4 (MacBook Pro)
- Ubuntu 18.04 and 19.04 (Intel Core i7 7th Generation, 16 Gb RAM, SSD 256)
In both OS, the installation of Miniconda3 is successful. However I'm
having issues with the installation of iGUIDE with both OS during the 1st
step (updating the environment of conda using /etc/requirements.yml.
In MacOS: gets stuck "Solving environment:" forever. Never finishes
in both distributions of Ubuntu, the process of solving environment gets
killed: "Solving environment: killed"
NOTE: I've tried to install iGUIDE in a freshly formated computer and with
both Ubuntu versions, updated and upgraded, right before starting the
installation.
I've checked if I was low in memory using the command $ free and there's
plenty. however when checking the CPU and memory usage with command $ top
after one point it takes over the 8 cores of the computer.
Do you have any suggestion on how to get the environment solved?
Thanks in advance.
Best,
Pedro.
San Mateo, CA, 94403
Email: pmendez00@gmail.com Skype: pmendez00 Phone: +1 (650) 398-6653
LinkedIn: https://www.linkedin.com/in/pedro-mendez-linkedi/
*This e-mail is privileged, confidential and contains private information.
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privilegiada. Si usted no es el destinatario del mensaje, o lo ha recibido
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…On Mon, Jun 15, 2020 at 5:09 AM Chris Nobles ***@***.***> wrote:
Alright, let's try an manual install. I should likely update the docs with
this process as well. If it's not clear, anything in the code blocks should
be input into the command line. My preferred installation has both the
miniconda and iGUIDE directories in my home directory.
Installing miniconda
Skip to installing iGUIDE if you already have miniconda or anaconda
installed. These can be executed in your home directory.
1. Get the latest version of miniconda install script.
__conda_url=https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
wget -q ${__conda_url} -O miniconda.sh
1. Installing miniconda through downloaded script. You can choose a
different path for the install, here it is installed into the home
directory.
__conda_path="~/miniconda3"
bash miniconda.sh -b -p ${__conda_path}
1. Source conda to activate the installation for use.
source ${__conda_path}/etc/profile.d/conda.sh
Installing iGUIDE
The following commands should be called from within the iGUIDE directory
(/path/to/iGUIDE/) after the repository is cloned. (If you need help on
this part, just let me know).
1. Install the conda environment from the requirements file. The name
field here can be changed to what you would like to call the environment,
default for the install script is 'iguide'.
conda env update --name=iguide --file etc/requirements.yml
1. After successful creation of the environment, activate the iguide
environment (or what you've called it).
conda activate iguide
1. Install the supporting R-package into the environment.
R CMD INSTALL tools/iguideSupport
1. Setup your environmental variables.
__iguide_dir=$(pwd)
echo -ne "#/bin/sh\nexport IGUIDE_DIR=${__iguide_dir}" > \
${CONDA_PREFIX}/etc/conda/activate.d/env_vars.sh
mkdir -p ${CONDA_PREFIX}/etc/conda/deactivate.d/
echo -ne "#/bin/sh\nunset IGUIDE_DIR" > \
${CONDA_PREFIX}/etc/conda/deactivate.d/env_vars.sh
1. Install the command line interface for iGUIDE using pip.
pip install --upgrade tools/iguidelib/
1. You should now deactivate and reactivate the environment to
initiate the environmental variables.
conda deactivate
conda activate iguide
Testing
Tests to make sure the components were installed correctly.
1. Test for required R-packages installed.
$(Rscript tools/rscripts/check_for_required_packages.R &> /dev/null) &&
echo true || echo false
1. Check to make sure iguideSupport was installed correctly.
$(Rscript tools/rscripts/check_pkgs.R iguideSupport &> /dev/null) && echo
true || echo false
1. Run unit tests for iguideSupport.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true
|| echo false'
1. Check to make sure the pip install of the CLI was successful.
command -v iguide &> /dev/null && echo true || echo false
1. Run tests for iGUIDE, this step will take a little bit to complete
if it starts processing the test case. Go grab a coffee, you deserve it
getting to this point. :) The test will activate the environment as part of
the test, so you should deactivate your environment first and then initiate
the test.
conda deactivate
bash etc/tests/test.sh iguide
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Really sorry about that, I made a mistake when I was writing the manual install earlier. I had you build the environment from the requirements file, but it's suppose to be built using the build file. This bypasses the environmental solving which can take some time. Try the updated install below and let me know how it goes. My preferred installation has both the miniconda and iGUIDE directories in my home directory. Installing minicondaSkip to installing iGUIDE if you already have miniconda or anaconda installed. These can be executed in your home directory.
Installing iGUIDEThe following commands should be called from within the iGUIDE directory (/path/to/iGUIDE/) after the repository is cloned. (If you need help on this part, just let me know).
TestingTests to make sure the components were installed correctly.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true || echo false'
|
Hi Chris,
No worries at all. That happens to all of us. Thanks for your support. Now
I can create the environment, install the R package, and setup the
environment variables. However, when I try to Install the command line
interface for iGUIDE using pip, I get the following error:
Any guidance? Thanks in advance!!
Cheers,
Pedro.
[image: image.png]
Pedro Mendez. San Mateo, CA, 94403 Email: pmendez00@gmail.com Skype:
pmendez00 Phone: +1 (650) 398-6653
LinkedIn: https://www.linkedin.com/in/pedro-mendez-linkedi/
*This e-mail is privileged, confidential and contains private information.
Any reading, retention, distribution or copy of this communication by any
person other than its intended recipient is prohibited.*
*Este mensaje electrónico puede contener información confidencial o
privilegiada. Si usted no es el destinatario del mensaje, o lo ha recibido
por error, por favor notifíquelo al remitente y destruya el mensaje con
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…On Thu, Jun 18, 2020 at 5:27 AM Chris Nobles ***@***.***> wrote:
Really sorry about that, I made a mistake when I was writing the manual
install earlier. I had you build the environment from the requirements
file, but it's suppose to be built using the build file. This bypasses the
environmental solving which can take some time. Try the updated install
below and let me know how it goes. My preferred installation has both the
miniconda and iGUIDE directories in my home directory.
Installing miniconda
Skip to installing iGUIDE if you already have miniconda or anaconda
installed. These can be executed in your home directory.
1. Get the latest version of miniconda install script.
__conda_url=https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
wget -q ${__conda_url} -O miniconda.sh
1. Installing miniconda through downloaded script. You can choose a
different path for the install, here it is installed into the home
directory.
__conda_path="~/miniconda3"
bash miniconda.sh -b -p ${__conda_path}
1. Source conda to activate the installation for use.
source ${__conda_path}/etc/profile.d/conda.sh
Installing iGUIDE
The following commands should be called from within the iGUIDE directory
(/path/to/iGUIDE/) after the repository is cloned. (If you need help on
this part, just let me know).
1. Install the conda environment from the requirements file. The name
field here can be changed to what you would like to call the environment,
default for the install script is 'iguide'. *This is the part that
changed from above.*
conda create --name=iguide --quiet --yes --file etc/build.b1.0.1.txt
1. After successful creation of the environment, activate the iguide
environment (or what you've called it).
conda activate iguide
1. Install the supporting R-package into the environment.
R CMD INSTALL tools/iguideSupport
1. Setup your environmental variables.
__iguide_dir=$(pwd)
echo -ne "#/bin/sh\nexport IGUIDE_DIR=${__iguide_dir}" > \
${CONDA_PREFIX}/etc/conda/activate.d/env_vars.sh
mkdir -p ${CONDA_PREFIX}/etc/conda/deactivate.d/
echo -ne "#/bin/sh\nunset IGUIDE_DIR" > \
${CONDA_PREFIX}/etc/conda/deactivate.d/env_vars.sh
1. Install the command line interface for iGUIDE using pip.
pip install --upgrade tools/iguidelib/
1. You should now deactivate and reactivate the environment to
initiate the environmental variables.
conda deactivate
conda activate iguide
Testing
Tests to make sure the components were installed correctly.
1. Test for required R-packages installed.
$(Rscript tools/rscripts/check_for_required_packages.R &> /dev/null) &&
echo true || echo false
1. Check to make sure iguideSupport was installed correctly.
$(Rscript tools/rscripts/check_pkgs.R iguideSupport &> /dev/null) && echo
true || echo false
1. Run unit tests for iguideSupport.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true
|| echo false'
1. Check to make sure the pip install of the CLI was successful.
command -v iguide &> /dev/null && echo true || echo false
1. Run tests for iGUIDE, this step will take a little bit to complete
if it starts processing the test case. Go grab a coffee, you deserve it
getting to this point. :) The test will activate the environment as part of
the test, so you should deactivate your environment first and then initiate
the test.
conda deactivate
bash etc/tests/test.sh iguide
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Take 3. So the reason for the pip installation of the CLI failing was due to the environmental variables (IGUIDE_DIR) not being initiated. In step 4, we copy these environmental variables into the startup script for the environment. Since we wrote the data into the script, we haven't sourced the script to make the variables present in the environment. Switching steps 5 and 6 will resolve this issue. As least I has in my hands, as I was able to reproduce an error when following the above method at the pip installation. Remeber, my preferred installation has both the miniconda and iGUIDE directories in my home directory. If you have everything still set up, just activate the environment again and try the CLI pip installation. Otherwise, try the below method. If it works out for everyone, then I'll put this last method in the documentation as a manual installation process. Installing minicondaSkip to installing iGUIDE if you already have miniconda or anaconda installed. These can be executed in your home directory.
Installing iGUIDEThe following commands should be called from within the iGUIDE directory (/path/to/iGUIDE/) after the repository is cloned. (If you need help on this part, just let me know).
TestingTests to make sure the components were installed correctly.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true || echo false'
|
Hi Chris,
It worked!! Thanks a lot.
I'll start running the sample dataset and hopefully we can soon start our
experiments with Cas9 edited cells.
I'll keep you posted on the progress!
Again thanks for your help and for putting together iguide!
Cheers,
Pedro.
Pedro Mendez. San Mateo, CA, 94403 Email: pmendez00@gmail.com Skype:
pmendez00 Phone: +1 (650) 398-6653
LinkedIn: https://www.linkedin.com/in/pedro-mendez-linkedi/
*This e-mail is privileged, confidential and contains private information.
Any reading, retention, distribution or copy of this communication by any
person other than its intended recipient is prohibited.*
*Este mensaje electrónico puede contener información confidencial o
privilegiada. Si usted no es el destinatario del mensaje, o lo ha recibido
por error, por favor notifíquelo al remitente y destruya el mensaje con
todo su contenido. Está completamente prohibida cualquier copia, uso o
distribución no autorizada del contenido de este mensaje.*
…On Sat, Jun 20, 2020 at 8:52 AM Chris Nobles ***@***.***> wrote:
Take 3. So the reason for the pip installation of the CLI failing was due
to the environmental variables (IGUIDE_DIR) not being initiated. In step 4,
we copy these environmental variables into the startup script for the
environment. Since we wrote the data into the script, we haven't sourced
the script to make the variables present in the environment. Switching
steps 5 and 6 will resolve this issue. As least I has in my hands, as I was
able to reproduce an error when following the above method at the pip
installation. Remeber, my preferred installation has both the miniconda and
iGUIDE directories in my home directory. If you have everything still set
up, just activate the environment again and try the CLI pip installation.
Otherwise, try the below method. If it works out for everyone, then I'll
put this last method in the documentation as a manual installation process.
Installing miniconda
Skip to installing iGUIDE if you already have miniconda or anaconda
installed. These can be executed in your home directory.
1. Get the latest version of miniconda install script.
__conda_url=https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
wget -q ${__conda_url} -O miniconda.sh
1. Installing miniconda through downloaded script. You can choose a
different path for the install, here it is installed into the home
directory.
__conda_path="~/miniconda3"
bash miniconda.sh -b -p ${__conda_path}
1. Source conda to activate the installation for use.
source ${__conda_path}/etc/profile.d/conda.sh
Installing iGUIDE
The following commands should be called from within the iGUIDE directory
(/path/to/iGUIDE/) after the repository is cloned. (If you need help on
this part, just let me know).
1. Install the conda environment from the requirements file. The name
field here can be changed to what you would like to call the environment,
default for the install script is 'iguide'. *This is the part that
changed from above.*
conda create --name=iguide --quiet --yes --file etc/build.b1.0.1.txt
1. After successful creation of the environment, activate the iguide
environment (or what you've called it).
conda activate iguide
1. Install the supporting R-package into the environment.
R CMD INSTALL tools/iguideSupport
1. Setup your environmental variables.
__iguide_dir=$(pwd)
echo -ne "#/bin/sh\nexport IGUIDE_DIR=${__iguide_dir}" > \
${CONDA_PREFIX}/etc/conda/activate.d/env_vars.sh
mkdir -p ${CONDA_PREFIX}/etc/conda/deactivate.d/
echo -ne "#/bin/sh\nunset IGUIDE_DIR" > \
${CONDA_PREFIX}/etc/conda/deactivate.d/env_vars.sh
1. You should now deactivate and reactivate the environment to
initiate the environmental variables.
conda deactivate
conda activate iguide
1. Lastely, install the command line interface for iGUIDE using pip.
pip install --upgrade tools/iguidelib/
Testing
Tests to make sure the components were installed correctly.
1. Test for required R-packages installed.
$(Rscript tools/rscripts/check_for_required_packages.R &> /dev/null) &&
echo true || echo false
1. Check to make sure iguideSupport was installed correctly.
$(Rscript tools/rscripts/check_pkgs.R iguideSupport &> /dev/null) && echo
true || echo false
1. Run unit tests for iguideSupport.
`$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo true
|| echo false'
1. Check to make sure the pip install of the CLI was successful.
command -v iguide &> /dev/null && echo true || echo false
1. Run tests for iGUIDE, this step will take a little bit to complete
if it starts processing the test case. Go grab a coffee, you deserve it
getting to this point. :) The test will activate the environment as part of
the test, so you should deactivate your environment first and then initiate
the test.
conda deactivate
bash etc/tests/test.sh iguide
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Hi Chris,
Today, I wanted to get some files to play around with iGuide and I got
stuck in the first step.
apparently is having issues exiting from iguide. Any guidance?
Thanks!
Pedro.
[image: image.png]
Pedro Mendez. San Mateo, CA, 94403 Email: pmendez00@gmail.com Skype:
pmendez00 Phone: +1 (650) 398-6653
LinkedIn: https://www.linkedin.com/in/pedro-mendez-linkedi/
*This e-mail is privileged, confidential and contains private information.
Any reading, retention, distribution or copy of this communication by any
person other than its intended recipient is prohibited.*
*Este mensaje electrónico puede contener información confidencial o
privilegiada. Si usted no es el destinatario del mensaje, o lo ha recibido
por error, por favor notifíquelo al remitente y destruya el mensaje con
todo su contenido. Está completamente prohibida cualquier copia, uso o
distribución no autorizada del contenido de este mensaje.*
…On Fri, Jun 26, 2020 at 11:10 PM Pedro Mendez ***@***.***> wrote:
Hi Chris,
It worked!! Thanks a lot.
I'll start running the sample dataset and hopefully we can soon start our
experiments with Cas9 edited cells.
I'll keep you posted on the progress!
Again thanks for your help and for putting together iguide!
Cheers,
Pedro.
Pedro Mendez. San Mateo, CA, 94403 Email: ***@***.*** Skype:
pmendez00 Phone: +1 (650) 398-6653
LinkedIn: https://www.linkedin.com/in/pedro-mendez-linkedi/
*This e-mail is privileged, confidential and contains private information.
Any reading, retention, distribution or copy of this communication by any
person other than its intended recipient is prohibited.*
*Este mensaje electrónico puede contener información confidencial o
privilegiada. Si usted no es el destinatario del mensaje, o lo ha recibido
por error, por favor notifíquelo al remitente y destruya el mensaje con
todo su contenido. Está completamente prohibida cualquier copia, uso o
distribución no autorizada del contenido de este mensaje.*
On Sat, Jun 20, 2020 at 8:52 AM Chris Nobles ***@***.***>
wrote:
> Take 3. So the reason for the pip installation of the CLI failing was due
> to the environmental variables (IGUIDE_DIR) not being initiated. In step 4,
> we copy these environmental variables into the startup script for the
> environment. Since we wrote the data into the script, we haven't sourced
> the script to make the variables present in the environment. Switching
> steps 5 and 6 will resolve this issue. As least I has in my hands, as I was
> able to reproduce an error when following the above method at the pip
> installation. Remeber, my preferred installation has both the miniconda and
> iGUIDE directories in my home directory. If you have everything still set
> up, just activate the environment again and try the CLI pip installation.
> Otherwise, try the below method. If it works out for everyone, then I'll
> put this last method in the documentation as a manual installation process.
> Installing miniconda
>
> Skip to installing iGUIDE if you already have miniconda or anaconda
> installed. These can be executed in your home directory.
>
> 1. Get the latest version of miniconda install script.
>
> __conda_url=https://repo.continuum.io/miniconda/Miniconda3-latest-Linux-x86_64.sh
> wget -q ${__conda_url} -O miniconda.sh
>
>
> 1. Installing miniconda through downloaded script. You can choose a
> different path for the install, here it is installed into the home
> directory.
>
> __conda_path="~/miniconda3"
> bash miniconda.sh -b -p ${__conda_path}
>
>
> 1. Source conda to activate the installation for use.
>
> source ${__conda_path}/etc/profile.d/conda.sh
> Installing iGUIDE
>
> The following commands should be called from within the iGUIDE directory
> (/path/to/iGUIDE/) after the repository is cloned. (If you need help on
> this part, just let me know).
>
> 1. Install the conda environment from the requirements file. The name
> field here can be changed to what you would like to call the environment,
> default for the install script is 'iguide'. *This is the part that
> changed from above.*
>
> conda create --name=iguide --quiet --yes --file etc/build.b1.0.1.txt
>
> 1. After successful creation of the environment, activate the iguide
> environment (or what you've called it).
>
> conda activate iguide
>
> 1. Install the supporting R-package into the environment.
>
> R CMD INSTALL tools/iguideSupport
>
> 1. Setup your environmental variables.
>
> __iguide_dir=$(pwd)
>
> echo -ne "#/bin/sh\nexport IGUIDE_DIR=${__iguide_dir}" > \
> ${CONDA_PREFIX}/etc/conda/activate.d/env_vars.sh
>
> mkdir -p ${CONDA_PREFIX}/etc/conda/deactivate.d/
>
> echo -ne "#/bin/sh\nunset IGUIDE_DIR" > \
> ${CONDA_PREFIX}/etc/conda/deactivate.d/env_vars.sh
>
>
> 1. You should now deactivate and reactivate the environment to
> initiate the environmental variables.
>
> conda deactivate
> conda activate iguide
>
>
> 1. Lastely, install the command line interface for iGUIDE using pip.
>
> pip install --upgrade tools/iguidelib/
> Testing
>
> Tests to make sure the components were installed correctly.
>
> 1. Test for required R-packages installed.
>
> $(Rscript tools/rscripts/check_for_required_packages.R &> /dev/null) &&
> echo true || echo false
>
> 1. Check to make sure iguideSupport was installed correctly.
>
> $(Rscript tools/rscripts/check_pkgs.R iguideSupport &> /dev/null) && echo
> true || echo false
>
> 1. Run unit tests for iguideSupport.
>
> `$(Rscript tools/rscripts/check_iguideSupport.R &> /dev/null) && echo
> true || echo false'
>
> 1. Check to make sure the pip install of the CLI was successful.
>
> command -v iguide &> /dev/null && echo true || echo false
>
> 1. Run tests for iGUIDE, this step will take a little bit to complete
> if it starts processing the test case. Go grab a coffee, you deserve it
> getting to this point. :) The test will activate the environment as part of
> the test, so you should deactivate your environment first and then initiate
> the test.
>
> conda deactivate
> bash etc/tests/test.sh iguide
>
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Hi Christopher,
Thanks for putting iGUIDE together. This is great. While trying to install it has some issues creating the 'iguide' environment. See the screenshot attached. I'm looking forward to giving it a try this week and I would appreciate very much if you could help me out.
Thanks in advance for your time and help!!
Best regards,
Pedro Mendez.
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