yoRISC

A bit of code to interconvert objects between the Seurat and RISC environments for scRNA-seq data analysis. This source was mainly compiled by following the RISC guide to convert Seurat object to RISC object and getting insight from looking at the source code of the Seurat package (mainly on how their IntegrateData and RunPCA methods would work). The code was tested with the main functionality of Seurat for a generic scRNA-seq analysis. To run the conversion functions as presented in the conversion.R script you need the following packages

• RISC 1.0.0
• SeuratDisk 0.0.0.9019
• SeuratObject 4.0.0
• Seurat 4.0.1

For any additonal code presented in the risc_integration.Rmd you also have to install

• gridExtra 2.3
• ggplot2 3.3.3
• dplyr 1.0.5
• tidyverse 1.3.1
• BiocManager 1.30.12
• celldex 1.0.0
• pheatmap 1.0.12
• SingleR 1.4.1