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README
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README
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usage: [BAM data stream] | ./ogap [options]
Realigns alignments meeting specified criteria (number of gaps, mismatches) using
Smith-Waterman parameters optimized to open gaps and eliminate mismatches and
writes the stream of alignments as BAM on stdout.
arguments:
-f --fasta-reference FILE FASTA reference file to use for realignment (required)
-d --debug Print debugging information about realignment process
-w --flanking-window BP The number of bases on the left and right to attempt to
align to (default 300bp).
-c --max-position-delta Maximum number of bases the start or end of the alignment
may change when realigning (default 200bp)
-m --match-score The match score (default 10.0)
-n --mismatch-score The mismatch score (default -20.0)
-g --gap-open-penalty The gap open penalty (default 15.0)
-e --gap-extend-penalty The gap extend penalty (default 0.0)
-s --suppress-output Don't output BAM on stdout