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I'm currently using CNVkit for calling germline CNVs in Y chromosomes using WGS data.
For my input bams, I extracted chrY region of WGS data using samtools and converted into .bam format.
I have also added a target bed file (-t) only to cover chrY regions.
The tool runs fine although it returns a log message 'No chrX found in sample; is the input truncated?' as there is no chrX data.
I was wondering if missing chrX or other autosomal regions in input affects the log2 ratio calculation?
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Hello,
I'm currently using CNVkit for calling germline CNVs in Y chromosomes using WGS data.
For my input bams, I extracted chrY region of WGS data using samtools and converted into .bam format.
I have also added a target bed file (-t) only to cover chrY regions.
The tool runs fine although it returns a log message 'No chrX found in sample; is the input truncated?' as there is no chrX data.
I was wondering if missing chrX or other autosomal regions in input affects the log2 ratio calculation?
Thank you!
Subin
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