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PrimerCut is a tool for differentially cutting the primer sequence in aligned reads depending on where the primer sequence derived from (genomic DNA or PCR primers). REQUIREMENTS ------------ To use PrimerCut you need * ruby 1.9 * ruby gems: * bio * bio-samtools USING ----- Usage: PrimerCut [regions] -i, --input FILE Input BAM -o, --output FILE Output BAM -r, --regions [a,b,c or regions.bed] Regions to remove E.g.: PrimerCut -i source.bam -o target.bam -r 1:1070-1100,4:4500-4530 PrimerCut -i source.bam -o target.bam -r regions.bed LINKS ----- ftp://rogaevlab.ru/PrimerCut http://github.com/gusevfe/primercut
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Tool for removing primer sequences from aligned data
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