guanaco

Python library for doing 3D CTF correction

Installation

To install from source, clone this repository and then do the following:

git submodule update --init --recursive

In order to build this package, the following dependencies are required:

• The CUDA toolkit
• FFTW

pip install -r requirements.txt
python setup.py install 

Testing

To run the tests, clone this repository and the do the following:

python setup.py test

Usage

To do a tomographic reconstruction with no CTF correction do something do the following

guanaco -i images.mrc -o rec.mrc -d gpu

To correct all images with the same single defocus something do the following

guanaco -i images.mrc -o rec.mrc -d gpu --df=20000 --Cs=2.7

To correct all images with the same defocus range something do the following

guanaco -i images.mrc -o rec.mrc -d gpu --df=20000 --Cs=2.7 --ndf=10

Issues

Please use the GitHub issue tracker to submit bugs or request features.

License

Copyright Diamond Light Source and Rosalind Franklin Institute, 2021

Distributed under the terms of the GPLv3 license, guanaco is free and open source software.