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Before using the command:
gfatools view -l 303726,303738 -r 20 Assembly-BothStrands.gfa > Assembly-BothStrands_303726-303738_r20.gfa
my GFA has the following elements:
S 5924062 GTTTCAAAAAAAAAAAAAGAGCATGGCTCTGG RC:i:400 L 5209751 + 5924062 + 18M1D6M L 5420387 + 5924062 + 18M1D6M
After running the command, they look like this:
S 5924062 GTTTCAAAAAAAAAAAAAGAGCATGGCTCTGG LN:i:32 RC:i:400 L 5209751 + 5924062 + 24M L1:i:33 L2:i:8 L 5420387 + 5924062 + 24M L1:i:33 L2:i:8
These overlap Cigars are incorrect:
Cigar: 24M 5924062 1 GTTTCAAAAAAAAAAAAAGAGCATGGCTCTGG ||||||||||||||||||||||||| 5420387 34 ACTGCATTCCAGCCTGGGTGATAGAGTGAGGCTGTTTCAAAAAAAAAAAAAAGAGCAT
Compared to a local alignment with Smith-Waterman:
Cigar: 5M1I19M 5924062 1 GTTTC-AAAAAAAAAAAAAGAGCATGGCTCTGG ||||| ||||||||||||||||||| 5420387 34 ACTGCATTCCAGCCTGGGTGATAGAGTGAGGCTGTTTCAAAAAAAAAAAAAAGAGCAT
The text was updated successfully, but these errors were encountered:
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Before using the command:
gfatools view -l 303726,303738 -r 20 Assembly-BothStrands.gfa > Assembly-BothStrands_303726-303738_r20.gfa
my GFA has the following elements:
After running the command, they look like this:
These overlap Cigars are incorrect:
Compared to a local alignment with Smith-Waterman:
The text was updated successfully, but these errors were encountered: