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masikol edited this page Mar 1, 2023
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Welcome to the CAGER-misc wiki!
Here you can find miscellaneous bioinformatic tools from Laboratory “The Center of Analytical and Genetic Engineering Research”.
List of tools with links to manuals:
- pub: a script for automatic picking up sequencing barcodes;
- dedupl-fastq: the script is designed for deduplication of fastq files;
- sum-up-snv: a script for counting coverage and sigle nucleotive variants at a single specified position in SAM/BAM file;
-
mean-qual: the script calculates mean quality of reads in
fastq
file(s); - most-freq-subseq: the script finds N most frequently occuring subsequences of given length for each sequence in fasta file;
-
NOS: the sript counts non-overalapping occurences of query sequence (and it's reverse complement "comrade") in
fasta
file(s); -
fasta-GC-content: the script calculates GC-content of each sequence in
fasta
file(s); -
fastq2fasta: the script converts
fastq
files tofasta
format; -
fastq-read-count: the script counts amount of reads in
fastq
file(s); -
find-seq: the script finds fasta record(s) in
fasta
file by given sequence header; -
dna-summary: the script collects basic information from
.dna
SPAdes contigs incontigs/
directory; -
packer-dna-to-fasta: the script packs
.dna
SPAdes contigs in 'contigs' directory to single multi-fasta file; - seqator: This script performs binning. It bins sequences (in fasta format) and sequence-containing files which have SPAdes-like headers and file names, respectively. It filters sequences by length or by contig coverage;
- combinator-FQ: genome assembly facilitation. This script is now moved to the separate repository: https://github.com/masikol/combinator-FQ;
- kromsatel: a tool for splitting chimeric nanopore amplicon reads. This script is now moved to the separate repository: https://github.com/masikol/kromsatel;