accelerated wtdbg2, which is based on the de novo assembler wtdbg2 on https://github.com/ruanjue/wtdbg2
CPU: 2 Intel Xeon E5-2680v4 CPUs,each with 14 hyper-threaded cores.
Memory: 256GB
Operation System: Red Hat 7.2
Compiler: gcc 4.8.3
All the different command lines of various datasets have already been integrated in run.sh, here only the download links are presented.
E.coli: http://www.cbcb.umd.edu/software/PBcR/data/selfSampleData.tar.gz
C.elegans: https://github.com/PacificBiosciences/DevNet/wiki/C.-elegans-data-set
D. melanogaster ISO1: https://www.ebi.ac.uk/ena/data/view/SRR6702603
Human NA24385: https://ftp-trace.ncbi.nlm.nih.gov/giab/ftp/data/AshkenazimTrio/HG002_NA24385_son/PacBio_CCS_15kb/
Human HG00733: https://www.ebi.ac.uk/ena/data/view/SRR7615963
To compile and install the a-wtdbg2, please use make
and make install
.
cd a-wtdbg2
make
make install
The command lines for assembly and subsequent consensus which is used for assessment by Quast is in run.sh, thus to run a-wtdbg2, please directly run run.sh.
./run.sh