PyMOL4Spliceosome: all spliceosome structures in one PyMOL session
Tip: the session now is very big and it might slow your computer. We recommend to open it one, remove unnecessary things for your analysis and save it as a new session.
Processed PyMOL session of cryo-em structures, see https://github.com/mmagnus/PyMOL4Spliceosome/releases to learn more about current stage of the project.
The structures superimposed based on the U6 RNA (if possible)
Colors and chain mapping based on pyMoL_colors-EMX.xlsx prepared by Eysmont and Magnus.
Not everything is perfect, expect some updates soon.
and read more about the tool used to obtain this session: https://github.com/mmagnus/rna-tools/blob/master/rna_tools/tools/PyMOL4RNA/README.md
Select all U6
Morph between two main conformations of U6
With Up Down with Righ-mouse Click
PyMOL>enable CWC15* PyMOL>disable CWC15* # select only some objects and save it to the file save /Users/magnus/Desktop/XXXX_triplex_zoom.pse, CXXX_P and U2_P and U6_P
Read more: https://pymolwiki.org/index.php/Enable
You can also download sessions for single steps prepared by the Nagai lab: https://www2.mrc-lmb.cam.ac.uk/groups/nagai/resources/
ReMedy-International Research Agenda Unit, Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland
M.M. was supported by the "Regenerative Mechanisms for Health-ReMedy" grant MAB/20172, carried out within the International Research Agendas Program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund.