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Structures of the spliceosome processed to be easily viewed with PyMOL
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README.md

README.md

PyMOL4Spliceosome: all spliceosome structures in one PyMOL session

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Tip: the session now is very big and it might slow your computer. We recommend to open it one, remove unnecessary things for your analysis and save it as a new session.

Processed PyMOL session of cryo-em structures, see https://github.com/mmagnus/PyMOL4Spliceosome/releases to learn more about current stage of the project.

Screen Shot 2019-09-13 at 9 28 20 AM

The structures superimposed based on the U6 RNA (if possible)

Colors and chain mapping based on pyMoL_colors-EMX.xlsx prepared by Eysmont and Magnus.

Not everything is perfect, expect some updates soon.

See https://github.com/mmagnus/PyMOL4Spliceosome/releases

and read more about the tool used to obtain this session: https://github.com/mmagnus/rna-tools/blob/master/rna_tools/tools/PyMOL4RNA/README.md

Select all U6

Morph between two main conformations of U6

Tips

With Up Down with Righ-mouse Click

Quick reference

PyMOL>enable CWC15*
PyMOL>disable CWC15*

# select only some objects and save it to the file
save /Users/magnus/Desktop/XXXX_triplex_zoom.pse, CXXX_P and U2_P and U6_P

Read more: https://pymolwiki.org/index.php/Enable

External

You can also download sessions for single steps prepared by the Nagai lab: https://www2.mrc-lmb.cam.ac.uk/groups/nagai/resources/

Acknowledge

ReMedy-International Research Agenda Unit, Centre of New Technologies, University of Warsaw, 02-097 Warsaw, Poland

M.M. was supported by the "Regenerative Mechanisms for Health-ReMedy" grant MAB/20172, carried out within the International Research Agendas Program of the Foundation for Polish Science co-financed by the European Union under the European Regional Development Fund.

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