Systematic review and meta-analysis as described in:

• Milou S.C. Sep, Elbert Geuze, Marian Joëls. Impaired learning, memory, and extinction in posttraumatic stress disorder: translational meta-analysis of clinical and preclinical studies. medRxiv 2021.07.19.21260790; doi: https://doi.org/10.1101/2021.07.19.21260790 [preprint]
• Sep, M.S.C., Geuze, E. & Joëls, M. Impaired learning, memory, and extinction in posttraumatic stress disorder: translational meta-analysis of clinical and preclinical studies. Transl Psychiatry 13, 376 (2023). https://doi.org/10.1038/s41398-023-02660-7

Index

_ README.md: an overview of the project
|___ data: data files used in the project
|___ processed_data: intermediate files from the analysis
|___ results: results of the analyses (data, tables, figures)
|___ R: contains all R-code in the project

TRACE: Meta-analysis of learning and memory in PTSD

Step 1: Prepare data

Merge datasets

• script: Merge data S1, S2, S3 to one file with prepare_merge.rmd.
• input: search 1 TRACE data_collection_search1.xlsx, search 2 TRACE data_collection_search2.xlsx, search 3 TRACE_data_collection_search3.xlsx
• output:TRACEmerged.RDS

Recode data

• script: Recode variables and add method codes with prepare_recode.rmd.
• input:TRACEmerged.RDS, TRACE_method_codes.xlsx
• output: TRACErecoded.RDS

Process QA data

• script: process QA data with prepare_QA.rmd
• input: TRACE_RoBQA_data.xlsx,
• output: TRACE_QA_animal.RData, TRACE_QA_human.RData, and RoB.jpeg (optional: RoB_clinical.jpeg, RoB_preclinical.jpeg)

Calculate effect sizes

• script: prepare data for analysis in prepare_effect_size_QA.rmd
• input: TRACErecoded.RDS, TRACE_QA_animal.RData, and TRACE_QA_human.RData
• output: TRACEprepared.RData (nb n=1647)

Step 2: Meta-regression Valence x Phase

• script: meta-regression Valence x Phase: meta_regression.rmd. This script uses meta_regression_influentials.r to calculate potential influential case and outliers
• input: TRACEprepared.RData
• output: datasets used in analyses: clinical.data.metaregression.RDS, preclinical.data.metaregression.RDS and results (main, diagnostics, sensitivity, graphs)
  • clinical: phase_valence_PTSD_clinical.csv, phase_valence_PTSD_clinical.tiff, funnel.colours.clinical.tiff, phase_valence_PTSD_clinical.FSN.csv, influentials.clinical.rds, sens.clinical.infout.csv, sens.mod.A.csv, sens.mod.B.csv, sens.mod.C.csv
  • preclinical: phase_valence_PTSD_preclinical.csv, phase_valence_PTSD_preclinical.tiff, funnel.colours.preclinical.tiff, phase_valence_PTSD_preclinical.FSN.csv, influentials.preclinical.rds, sens.preclinical.infout.csv, sens.mod.E.csv, sens.mod.D.csv, sens.mod.F.csv
  • figure: PTSD.clinical.preclinical.tiff

Step 3: MetaForest and MetaCART

• script: meta_forest_meta_cart.rmd
• input: TRACEprepared.RData
• output: data.explore.rds (NB: also used for descriptives table)
  • clinical: clinical.data.explorative.RDS, preclinical.data.explorative.RDS, fitted.clinicalMetaForest.RDS, metaforest_Clinical_convergence.tiff, metaforest_Clinical_varImportance.tiff, important_variables_clinical_metaforest.csv, metaforest_PD_clinical.tiff
  • preclinical: fitted.preclinicalMetaForest.RDS, metaforest_Preclinical_convergence.tiff, metaforest_Preclinical_varImportance.tiff, REtree.P.rds, metaCART.preclinical.tiff, important_variables_preclinical_metaforest.csv, metaforest_PD_preclinical.tiff, metaforest_PD_preclinical_metaCARTfollowup.tiff, VarImp.clinical.preclinical.tiff

Step 4: Vizualization and Study descriptives

Flowchart

• script: flowchart.rmd
• input: PMID hits & screening search 1, 2 and 3: Review PTSD Cognition AbstractScreening_Overeenstemming EG & MS v15.11.2016.xlsx, pubmed_result_search2_human 6.1.20.txt, pubmed_result_search2_animal 6.1.20.txt, pmid.human.s3.learn.22.5.20.txt, pmid.animal.s3.learn.22.5.20.txt, Checked_TRACE_screening_search3_MS_EG.xlsx
• some additional code for screening in search 2
  • step 1: identify inconsistencies in search 2. input (initial screening data): TRACE_screening_search2_SH.xlsx & TRACE_screening_search2_MS.xlsx; output (abstract screening inconsistencies): inconsistencies_human_s2.csv & inconsistencies_animal_s2.csv
  • step 2: identify required full text checks in search 2. input (files with abstract screening feedback): inconsistencies_animal_s2_SH.csv & inconsistencies_human_s2_SH.csv"; output (uploaded to OSF): required_full_checks_human_s2.csv & required_full_checks_animal_s2.csv
  • step 3: get results full text check search 2. input (files with full text screening feedback): required_full_checks_animal_s2_MS.csv" & required_full_checks_human_s2_SH.csv"; output (uploaded to OSF): animal_inclusions_s2.csv & human_inclusions_s2.csv
• input: Data extraction information: TRACE data_collection_search1.xlsx, TRACE data_collection_search2.xlsx, TRACE_data_collection_search3.xlsx
• input: analyses datasets (NB output analyses scripts): TRACEprepared.RData, clinical.data.metaregression.RDS, clinical.data.explorative.RDS", preclinical.data.metaregression.RDS, preclinical.data.explorative.RDS
• output: unique_screened_articles.csv. en flowchart.tiff
• optional output (used to select papers for QA): Human.PMIDs.QA.S2.csv, Animal.PMIDs.QA.S2.csv. en Human.PMIDs.QA.S3.csv en Animal.PMIDs.QA.S3.csv

Characteristics of the included studies

• Script: descriptives.rmd
• input: data.explore.rds, created in DataDrivenAnalysis.rmd
• output: tables in word files: descriptives.comparison.doc, descriptives.csv (optional), descriptives.combined.doc, descriptives.clinical.doc, descriptives.preclinical.doc

Visualize QA

• script: visualize_QA.Rmd
• input: TRACE_QA_animal.RData, TRACE_QA_human.RData, clinical.data.metaregression.RDS preclinical.data.metaregression.RDS (Note: these objects are outputs from prepare_QA.RMD and meta_regression.rmd)
• output: RoB.jpeg