Skip to content
New issue

Have a question about this project? Sign up for a free GitHub account to open an issue and contact its maintainers and the community.

By clicking “Sign up for GitHub”, you agree to our terms of service and privacy statement. We’ll occasionally send you account related emails.

Already on GitHub? Sign in to your account

salmon_summarizedexperiment.r failing with test data #940

Closed
svenbioinf opened this issue Feb 7, 2023 · 1 comment
Closed

salmon_summarizedexperiment.r failing with test data #940

svenbioinf opened this issue Feb 7, 2023 · 1 comment
Assignees
Labels
bug Something isn't working

Comments

@svenbioinf
Copy link

Description of the bug

Hello,
I am running into an error using the latest nf-core/rnaseq pipeline with test-data from the test-datasets repository. Below is an reproducible example:

Command used and terminal output

nextflow run nf-core/rnaseq --outdir out/ --input https://raw.githubusercontent.com/nf-core/test-datasets/rnaseq/samplesheet/v3.10/samplesheet_test.csv 
--skip_alignment --pseudo_aligner salmon -profile singularity 
--max_cpus 8 --max_memory 40.GB 
--transcript_fasta https://github.com/nf-core/test-datasets/raw/rnaseq/reference/transcriptome.fasta 
--fasta https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genome.fasta 
--gtf https://github.com/nf-core/test-datasets/raw/rnaseq/reference/genes.gtf.gz
Error:
ColData not avaliable NULL
  Error in rbind(deparse.level, ...) : 
    numbers of columns of arguments do not match
Caused by:
  Process `NFCORE_RNASEQ:RNASEQ:QUANTIFY_SALMON:SALMON_SE_TRANSCRIPT (salmon_tx2gene.tsv)` terminated with an error exit status (1)

Command executed:

  salmon_summarizedexperiment.r \
      NULL \
      salmon.merged.transcript_counts.tsv \
      salmon.merged.transcript_tpm.tsv

Relevant files

No response

System information

nf-core/rnaseq v3.10.1-g6e1e448
N E X T F L O W ~ version 22.10.6
Singularity
Linux
Ubuntu 22.04

@svenbioinf svenbioinf added the bug Something isn't working label Feb 7, 2023
@MatthiasZepper MatthiasZepper self-assigned this Feb 8, 2023
@drpatelh
Copy link
Member

drpatelh commented Feb 8, 2023

Thanks for reporting @svenbioinf ! @MatthiasZepper has fixed this for you on the dev branch if you would like to test.

nextflow pull nf-core/rnaseq -r dev
nextflow run <YOUR_ORIGINAL_COMMAND> -r dev

Will close this for now but feel free to re-open if the problem persists.

@drpatelh drpatelh closed this as completed Feb 8, 2023
Sign up for free to join this conversation on GitHub. Already have an account? Sign in to comment
Labels
bug Something isn't working
Projects
None yet
Development

No branches or pull requests

3 participants