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Add support for sequencing coverage esimates with nonpareil #384

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jfy133 opened this issue Sep 27, 2023 · 4 comments
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Add support for sequencing coverage esimates with nonpareil #384

jfy133 opened this issue Sep 27, 2023 · 4 comments
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enhancement Improvement for existing functionality

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@jfy133
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jfy133 commented Sep 27, 2023

Description of feature

Main module here: nf-core/modules#3885

Might be nice to also make a MultiQC module for rendering curves.

Important: requires uncompressing FASTQ files for input! will need to give big warnings about disk space on this.

@jfy133 jfy133 added the enhancement Improvement for existing functionality label Sep 27, 2023
@jfy133 jfy133 self-assigned this Oct 25, 2023
@jfy133 jfy133 added this to the 1.2.0 - Bouncy Basenji milestone Oct 25, 2023
@jfy133
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jfy133 commented Oct 25, 2023

Maybe should update the module to conditionally unzip within it, rather than expected it outside

@Midnighter
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I was thinking, it would be awesome to have a QC step that shows an overview of where classified reads map on the identified reference genomes. Can nonpareil generally do that or does it only deliver estimates on how much coverage was achieved?

@jfy133
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jfy133 commented Oct 25, 2023

No, it purely aligns reads to themselves to check for redundancy essentially.

I'm not really sure there is a tool that does what you're asking for at a metagenomic level...

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jfy133 commented Dec 1, 2023

Done in in the pr that GitHub mobile is not finding stm

@jfy133 jfy133 closed this as completed Dec 1, 2023
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